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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (3): 636-645.DOI: 10.16409/j.cnki.2095-039x.2023.02.020

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Screening, Identification and Antagonistic Effect of Streptomyces against Phytophthora sansomeana

LI Wenqiao, WANG Jiao, LIU Xiaoning, LI Yaning, HE Yunzhuan, LIU Daqun   

  1. National Engineering Research Center for Agriculture in Northern Mountainous Areas/Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province/College of Plant Protection, Hebei Agricultural University, Baoding 071001, China
  • Received:2022-09-20 Online:2023-06-08 Published:2023-06-25

Abstract: To provide effective biological control resources for the green control of P. sansomeana, Streptomyces strains were isolated and screened with antagonistic effects against P. sansomeana from rhizosphere soil of healthy atractylodes plants in the area of P. sansomeana in Anguo of Hebei province. As results, three antagonistic Streptomyces strains (BZX-104, BZX-23 and BZX-31) with good antagonistic effect against P. sansomeana were isolated and screened by dilution coating method and plate confrontation method. After 16S rDNA sequence analysis, combined with colony morphological characteristics, and physiological and biochemical characteristics, the three antagonistic strains were identified as S. alfalfae, S. galilaeus and S. bobili, respectively. Their inhibitory activity against P. sansomeana were more than 60%.

Key words: P. sansomeana, antagonistic Streptomycetes, 16S rDNA sequence analysis, bacteriostatic effect

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