Identification of Streptomyces and Its Metabolites Antagonizing Fusarium oxysporum and Phytophthora parasitica in Tobacco Based on Non-Target Metabolomics

doi:10.16409/j.cnki.2095-039x.2023.02.036

Abstract

Abstract: To develop effective biocontrol products against two soil-borne diseases, root rot (Fusarium oxysporum) and black shank (Phytophthora parasitica), a Streptomyces strain with good control effect was screened by our laboratory from tobacco rhizosphere soil. It was identified as Streptomyces diastatochromogenes by morphological, physiological, biochemical characteristics and molecular biological analysis. The main active metabolite of the biocontrol Streptomyces was identified as anisomycin by LC/MS non-target metabolomics analysis. The product was verified and content determined by high performance liquid chromatography, and it was found that anisamycin contained 423.98 µg per gram of crude extract. The inhibition results on mycelial morphology and mycelial growth showed that anisomycin could cause the mycelia of F. oxysporum and P. parasitica to expand and distort. The EC₅₀ values for F. oxysporum and P. parasitica were 1556.36 mg/mL and 40.74 mg/mL, respectively. This study provides a new source of biocontrol agents for tobacco root rot and black shank diseases, and provided valuable insights into the further use of anisomycin.

Key words: Fusarium oxysporum, Phytophthora parasitica, Streptomyces diastatochromogenes, LC/MS non-target metabolomics, anisomycin

CLC Number:

S476

LIU He, SHAN Yuhang, QIU Rui, LI Shujun, ZHANG Chong, WU Yuanhua, AN Mengnan. Identification of Streptomyces and Its Metabolites Antagonizing Fusarium oxysporum and Phytophthora parasitica in Tobacco Based on Non-Target Metabolomics[J]. Chinese Journal of Biological Control, 2023, 39(4): 875-884.

References

[1] 方文生, 曹坳程, 韩大伟, 等. 两种熏蒸剂对土传病害的防控效果及黄瓜产量的影响[J]. 中国蔬菜, 2016, 7:44-48.
[2] 易龙, 邱妙文, 陈永明, 等. 烟草黑胫病的生物防治研究进展[J]. 中国农学通报, 2017, 33(25):146-151.
[3] 潘淑媛. 分离纯化方法对微生物药品质量的影响分析[J]. 数理医药学杂志, 2015, 28(7):1024-1025.
[4] 章智钧, 刘怀锋, 孙军利, 等. 非靶向代谢组学对赤霞珠果皮不同砧穗组合差异代谢物的分析[J]. 食品科学, 2020, 41(24):22-30.
[5] Benton H P, Ivanisevic J, Mahieu N G, et al. Autonomous metabolomics for rapid metabolite identification in global profiling[J]. Analytical Chemistry, 2015, 87(2):884-891.
[6] Plumb R S, Granger J H, Stumpf C L, et al. A rapid screening approach to metabonomics using UPLC and oa-TOF mass spectrometry:application to age, gender, and diurnal variation in normal/Zucker obese rats and black, white and nude mice[J]. The Analyst, 2005, 130(6):844-849.
[7] 司洪阳. 杀真菌链霉菌(Streptomyces fungicidicus)代谢产物的分离纯化及结构鉴定[D]. 沈阳:沈阳农业大学, 2019.
[8] 郭霞凌, 李辉. 一种发酵生产茴香霉素的方法及培养基[P]. 中国国发明专利, CN110257449A, 2019.
[9] 豆永辉, 李小聪, 刘国际, 等. 高效液相色谱外标法测定反应液中2-甲基萘和β-甲萘醌的含量[J]. 理化检验(化学分册), 2019, 55(12):1460-1462.
[10] 李亚南, 饶敏, 魏维, 等. 始旋链霉菌HCCB10218产生的诺卡胺类物质的发现[J]. 中国抗生素杂志, 2015, 40(6):414-418.
[11] 颜华, 赵康康, 张媛, 等. 密旋链霉菌Act12中调控因子AdpA-s和LuxR-2306对寡霉素合成的影响[J]. 中南民族大学学报(自然科学版), 2022, 41(4):418-424.
[12] Nóbile M L, Stricker A M, Iribarren A M, et al. Streptomyces griseus:A new biocatalyst with N-oxygenase activity[J]. Journal of Biotechnology, 2021, 327:36-42.
[13] 徐亦雯, 张诺, 曹瑱艳, 等. 浙麦冬黑斑病病原菌的分离鉴定及淀粉酶产色链霉菌对其生物防治效果[J]. 中国生物防治学报, 2022, 38(5):1280-1287.
[14] 曹瑱艳, 申屠旭萍, 俞晓平. 杭白菊枯萎病的病原菌分离鉴定及淀粉酶产色链霉菌对其防治效果[J]. 中国生物防治学报, 2019, 35(2):265-271.
[15] Ahsan T. 烟草靶斑病菌拮抗链霉菌的筛选、抗菌物质的鉴定及作用机制研究[D]. 沈阳:沈阳农业大学, 2017.
[16] 聂菊. 阿波霉素产生菌-灰色链霉菌ATCC 700974的自我保护机制研究[D]. 重庆:西南大学, 2021.
[17] Tengstrand E, Rosén J, Hellenäs K E, et al. A concept study on non-targeted screening for chemical contaminants in food using liquid chromatography-mass spectrometry in combination with a metabolomics approach[J]. Analytical and Bioanalytical Chemistry, 2013, 405(4):1237-1243.
[18] Cajka T, Danhelova H, Zachariasova M, et al. Application of direct analysis in real time ionization-mass spectrometry (DART-MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud[J]. Metabolomics, 2013, 9:545-557.
[19] Shen Y F, Hu Y, Zhu B, et al. Antiviral activity of anisomycin against spring viraemia of carp virus in epithelioma papulosum cyprini cells and zebrafish[J]. Virus Research, 2019, 268:38-44.
[20] 张晶, 崔宇琼, 夏焕章. 单组分茴香霉素C突变株的获得[J]. 沈阳药科大学学报, 2011, 28(8):616-620.
[21] Jiang M, Xu X, Song J, et al. Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea[J]. International Journal of Systematic and Evolutionary Microbiology, 2021, 71(9):e005004.
[22] Wu G, Robertson A J, Liu X, et al. A lipid transfer protein gene BG-14 is differentially regulated by abiotic stress, ABA, anisomycin, and sphingosine in bromegrass (Bromus inermis)[J]. Journal of Plant Physiology, 2004, 161(4):449-458.