Preliminary Study on The Control Mechanism of Pseudomonas fluorescens 2P24 on Bacterial Fruit Blotch

doi:10.16409/j.cnki.2095-039x.2023.02.022

Abstract

Abstract: In order to explore the mechanism of Pseudomonas fluorescens 2P24 against bacterial fruit blotch, the effects of the secondary metabolites, 2,4-DAPG and MPC on the growth of the pathogen, Acidovorax citrulli were tested. The disease control efficacy of 2P24 and its mutants ΔphlD (lack of 2,4-DAPG synthesis) and ΔgacS (lack of 2,4-DAPG, HCN and protease synthesis) were compared and analyzed. The results showed that 2,4-DAPG and MPC inhibited the growth, motility, and biofilm formation of A. citrulli. Compared with 2P24, the growth inhibition effect of △phlD decreased and ΔgacS lost. The relative control efficacy of ΔphlD and ΔgacS reduced by 44.3% and 77.1%, respectively, comparing to 2P24. 2P24 promoted the growth of watermelon and melon seedlings, increased the height and fresh weight of seedlings. In addition, 2P24 improved the activity of SOD, CAT and POD of melon and increased the expression of three resistance related genes, MELO3C023441, MELO3C022146, MELO3C004303. This study provided a reference and theoretical basis for the biological control of bacterial fruit blotch.

Key words: Acidovorax citrulli, bacterial fruit blotch, biocontrol bacteria, seed-borne disease

CLC Number:

S476

WANG Xinyu, LU Yu, QIU Yanhong, ZHANG Haijun, ZHANG Liqun, LI Jianqiang, WANG Hongyang, LUO Laixin, XU Xiulan. Preliminary Study on The Control Mechanism of Pseudomonas fluorescens 2P24 on Bacterial Fruit Blotch[J]. Chinese Journal of Biological Control, 2023, 39(3): 575-584.

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