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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (1): 204-211.DOI: 10.16409/j.cnki.2095-039x.2023.02.004

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Screening, Identification and Control Efficacy of Bacillus megaterium with Antagonist Activity against Ginger Wilt

WANG Qian1, SONG Juan1, LI Dongping1, QIN Xiaojuan2, CHEN Tingsu1, ZHOU Shengmao3   

  1. 1. Microbiology Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
    2. Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
    3. Vegetable Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2022-01-26 Online:2023-02-08 Published:2023-02-21

Abstract: Bacterial wilt of ginger, referred to as “ginger blast” caused by Ralstonia solanacearum, is the destructive pathogen of ginger reported from all the ginger growing countries. But there is no means available for effective control of the disease in field. Obtaining effective strains with disease control activity is an urgent prerequisite for biological control efficiency. This study was performed to explore if the plant growth-promoting rhizobacteria (PGPR) in ginger plants had any potential to induce resistance to Ralstonia solanacearum. Three strains were selected for evaluation of ginger growth promotion ability and control efficacy against the ginger wilt through culture dish and pot culture method, and the dominant strain YB-3 was identified based on its 16S rDNA sequence homology with the related bacteria from GenBank as well as physiological and biochemical characters. The results showed that the control effect of strain YB-3was 60.86% in pot experiment. The strain YB-3 was identified as Bacillus megaterium by morphological observations, physiological and biochemical tests, combined with 16S rDNA analysis. These results indicated that the strain YB-3 had the exploitation value against ginger wilt disease.

Key words: ginger wilt, biocontrol bacteria, biocontrol effect, Bacillus megaterium

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