Transcriptomic Analysis of the Effects of Beauveria bassiana Colonization on Maize Growth and Resistance under Elevated CO2

doi:10.16409/j.cnki.2095-039x.2024.11.008

Abstract

Abstract: The objective of this study was to clarify the underlying molecular mechanism of Beauveria bassiana colonization on plant growth and resistance under elevated CO₂. The study, employing maize B73 cultivar as the plant material and open top chambers for enrichment of CO₂, measured the growth and stress resistance of maize plants with B. bassiana colonization and explored the underlying mechanism through transcriptome sequencing and bioinformatics analysis. Transcriptome sequencing and fluorescence quantitative PCR confirmed that, when compared with normal atmospheric CO₂ concentration (Control), the increase of CO₂ concentration (eCO₂) and the colonization of B. bassiana (Bb) had significant effects on the expression of genes related to resistance and growth promotion in maize. The comparisons of Bb vs eCO₂+Bb, Control vs Bb, Control vs eCO₂, and eCO₂ vs eCO₂+Bb revealed 907, 803, 589, and 669 differentially expressed genes (DEGs), respectively, which included 407, 429, 360, and 238 genes with up-regulated expression and 500, 374, 229, and 431 genes with down-regulated expression, respectively. Among these DEGs, 16 were common in the pairwise comparisons of samples from different treatment groups. The DEGs related to plant growth and resistance were mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, glycosaminoglycan degradation, steroid biosynthesis, the pathways of diterpenoid biosynthesis and benzoxazinoid biosynthesis. The qRT-PCR verified the transcriptome analysis results of DEGs. The increase of CO₂ concentration and the colonization of B. bassiana have a positive regulatory effect on the functional genes and pathways related to maize growth and resistance to a certain extent, and then have a positive impact on the growth and development of maize plants.

Key words: climate change, entomopathogenic fungi, endophytic, growth promotion, transcriptome

CLC Number:

S476.1

WEN Huan, ZHANG Zhengkun, LU Yang, SUI Li, LI Qiyun. Transcriptomic Analysis of the Effects of Beauveria bassiana Colonization on Maize Growth and Resistance under Elevated CO₂[J]. Chinese Journal of Biological Control, 2025, 41(5): 1063-1077.

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Transcriptomic Analysis of the Effects of Beauveria bassiana Colonization on Maize Growth and Resistance under Elevated CO₂

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