Isolation,Identification and Evaluation of Insecticidal Activity of an Entomopathogenic Bacteria Strain Infecting Post-overwintering Ostrinia furnacalis Larvae

doi:10.16409/j.cnki.2095-039x.2024.01.002

Abstract

Abstract: A bacterial strain ACB3014 was isolated from the infected overwintering larvae of Ostrinia furnacalis in spring sown corn fields in Xinyuan County,Yili Valley,Xinjiang and identified by morphological observation,physiological and biochemical characteristics and 16S rDNA sequence analysis.The pathogenicity of this strain against the 3^rd instar larvae of O.furnacalis was determined by feeding method.The strain ACB3014 was Serratia marcescens.The cumulative corrected mortality of ACB3014 on the 3^rd instar larvae increased with the increase of bacterial suspension concentration.On the 7^th day after feeding ACB3014 bacterial suspension,the cumulative adjusted mortality of the 3^rd instar larvae ranged between 27.78%—77.78% at the concentration of 2× 10⁴—2× 10⁸ cfu/mL.On the 13th day of inoculation,when the concentration reached 2× 10⁸ cfu/mL,LC₅₀ and LT₅₀ against the 3^rd instar larvae of O.furnacalis were 3.79× 10³ cfu/mL and 3.93 days,respectively.In addition,the infected O.furnacalis larvae are dark red and black and are pyoid and festering,giving off a strong odor.In conclusion,ACB3014 strain has good insecticidal activity against O.furnacalis larvae and has a high potential as a green biological control resource for O.furnacalis.

Key words: Ostrinia furnacalis, Serratia marcescens, determination of virulence, pathogenic, insecticidal activity

CLC Number:

S476.2

YUAN Zihan, WANG Xiaowu, DING Xinhua, FU Kaiyun, JIANG Xudong, GONG Xuehua, JIA Zunzun, TOULXUN·Ahemaiti, GUO Wenchao. Isolation,Identification and Evaluation of Insecticidal Activity of an Entomopathogenic Bacteria Strain Infecting Post-overwintering Ostrinia furnacalis Larvae[J]. Chinese Journal of Biological Control, 2024, 40(2): 310-318.

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