Epidemic and Genetic Diversity of Beauveria bassiana chrysovirus 2

doi:10.16409/j.cnki.2095-039x.2023.01.048

Abstract

Abstract: Beauveria bassiana, an important entomogenous fungus, is widely used in biological control of pests. However, the pathogenicity degradation is an important factor restricting its application. Mycovirus could affect the pathogenicity of host fungi. A mycovirus Beauveria bassiana chrysovirus2 (BbCV2) reduces the virulence of host fungi B. bassiana population in the field. With the aim to understand the epidemic of the mycovirus, the infection of mycovirus BbCV2 in strains of B. bassiana isolated from Ostrinia furnacalis collected from 7 different sites in Jilin Province was verified by dsRNA extraction and RT-PCR methods, and the genetic diversity of BbCV2 was analyzed. The results showed that the infection of dsRNA mycovirus, especially the BbCV2, were universal in all samples, with the BbCV2 infection rate ranging from 70% to 100%. The genetic diversity of the RNA-dependent RNA polymerase (RdRp) gene of BbCV2 showed a highly conserved similarity, of which the nucleic acid sequence and amino acid sequence were up to 99.78% and 99.98%, respectively, indicating the virus is genetically stable. Further, the virus distribution and genetic evolution showed no relationship with geographical location. Therefore, the mycovirus BbCV2 is characterized by natural prevalence, which may have caused the pathogenicity degradation in the field. And B. bassiana adapts well to the mycovirus and exerts no strong selective pressure on the virus genetic evolution.

Key words: Beauveria bassiana, mycovirus, genetic evolution, biocontrol

CLC Number:

S476.2

LIU Hongyu, NIU Xinze, SUN Yang, ZHAO Yu, LU Yang, SUI Li, LI Qiyun, ZHANG Zhengkun. Epidemic and Genetic Diversity of Beauveria bassiana chrysovirus 2[J]. Chinese Journal of Biological Control, 2024, 40(3): 592-599.

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