Screening and Identification of Biocontrol Fungi against Fusarium Wilt of Banana and Evaluation of Their Control Effect

doi:10.16409/j.cnki.2095-039x.2025.02.061

Abstract

Abstract: To explore the biocontrol strains with good control effects on banana Fusarium wilt disease, forty five strains of fungi were isolated from forest soil and banana plantation soil in this study. Using the plate confrontation method, nine strains with good inhibitory effects on Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) were obtained. All of nine showed an inhibition rate exceeding 64%. Under the scanning electron microscope, it was observed that six strains caused Foc TR4 to exhibit morphological abnormalities, including shrunken and desiccated hyphae as well as deformed spores. However, the remaining three strains had no observable effect on Foc TR4 morphology. The greenhouse pot experiments were used to evaluate the control effect of 9 stains on banana Fusarium wilt disease, and the results showed that all nine strains had significant control effects. Strain HH16, HH19 and HH20 have the best control effect on banana Fusarium wilt disease, with the control efficacy greater than 64%. Morphological and molecular biological identification revealed the following results, strain BL01 as Trichoderma hamatum, BL02 as T. spirale, BL03 as T. lixii, BL04 as T. pinicola, BL05 as Penicillium raistrickii, BL08 as P. brefeldianum, HH16 as T. yunnanense, HH19 as T. crassum, and HH20 as T. virens. This study will provide theoretical basis and technical support for the development and application of biocontrol agents for banana Fusarium wilt disease.

Key words: banana Fusarium wilt, biological control, Trichoderma, Penicillium

CLC Number:

S476

YIN Shunli, MAO Jun, WANG Jielin, FENG Chencheng, DING Yue, SU Yuan, BAI Tingting. Screening and Identification of Biocontrol Fungi against Fusarium Wilt of Banana and Evaluation of Their Control Effect[J]. Chinese Journal of Biological Control, 2025, 41(5): 1188-1199.

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