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Screening and Breeding of Production Strains of Spinosad

HE Yu-ping, DAI Jing-yuan, DAI Peng, HU Xi-zhou, LIN Kai-chun   

  1. Wuhan Nature's Favour Bio-engineering Co., LTD, College of Plant Science and Technology, Huazhong Agricultural University 430070, China
  • Received:2005-01-24 Revised:1900-01-01 Online:2006-02-08 Published:2006-02-08
  • Contact: LIN Kai-chun

Abstract: Protoplast of Saccharopolyspora spinosa C121 was mutated by UV, then different dosages of plasma radiation and 60Co-γ irradiation were used to treat the high yielding strains respectively. The mutants were screened by strain blockTLC and shaking flask, eventually a mutant strain Co329 was obtained and its production was up to 1260μg/ml, 174% higher than that of the original strain C121.

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