Inhibitory Effect of the Yeast Volatile Compound Cyclooctatetraene on Botrytis cinerea

doi:10.16409/j.cnki.2095-039x.2017.02.018

Abstract

Abstract: In order to determine the inhibitory effect of volatile organic compounds produced by biocontrol yeast strain C410 onBotrytis cinerea, cyclooctatetraene, one of the dominant components of volatile organic compounds, was used to investigate its effect on sugar, protein, DNA, RNA, cell morphology and cell membrane permeability of B. cinerea. The results showed that when B. cinerea was treated with 16.5 μL/L cyclooctatetraene, the growth rate, the reducing sugar content and the RNA integrity were significantly decreased, while the total sugar content was barely changed and the protein content was declined slightly. When treated with 49.5 μL/L cyclooctatetraene, B. cinerea stopped growing, but the cell permeability was not affected and cells would not die. DNA was not destroyed by cyclooctatetraene. Therefore, the synthesis and metabolism of reducing sugars and RNA may be two important targets for cyclooctatetraene to suppress the pathogen growth and infection.

Key words: cyclooctatetraene, Botrytis cinerea, inhibition, mechanism

CLC Number:

S476.8

HUANG Rong, HU Jiankun, HUANG Ruirong. Inhibitory Effect of the Yeast Volatile Compound Cyclooctatetraene on Botrytis cinerea[J]. journal1, 2017, 33(2/3): 266-272.

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