Abstract: Bacillus methylotrophicu SHB114 was firstly isolated from deep-sea sediment collected from South China Sea. Three anti-fungal lipopeptides of the bacillomycin L_c family were purified from the fermentation broth via bioactivity-guided fractionation and isolation based on acid precipitation, vacuum flashchromatography, and semi-preparative HPLC. The three compounds were identified by interpretation of their NMR and MS spectroscopic data. All compounds showed significant inhibitory activities against fungal plant pathogens including Colletotrichum orbiculare, Rhizoctonia solani and Fusarium oxysporum f. sp. cucumerinum in paper-agar disk diffusion assay.