Abstract: We evaluated the colonization of B. subtilis Bs-15 in jujube (Zizyphus jujuba Mill. ‘Jinsi 4’) plants and its rhizospheric soil via pot experiments, and measured its effects on the community structure and functional diversity of soil microbes. Results showed that the Bs-15 colonized well in jujube plant and soil by irrigating and leaf daubing, with no less than 10⁴ cfu/g of colonization amount. The number of bacteria and actinomycetes in soil increased but without significance, while that of fungi decreased significantly after Bs-15 inoculation. The Biolog ECO microplates analysis showed that averaged well color development (AWCD) value of the microbial communities in the Bs-15 inoculated soil increased a little before 72 h, and increased significantly after 72 h. In the presence of Bs-15, the parameters (Shannon index, Simpson index and McIntosh index) of the functional diversity of the soil microbial communities increased significantly at 0.01 level, and Shannon uniformity and McIntosh uniformity increased significantly at 0.05 level.