Abstract: We investigated the synergistic effects of soil fumigation with Dazomet and biocontrol fungus Clonostachys rosea 67-1 used successively for control of cucumber fusarium wilt in protected fields. Pot experiments showed that death rate of cucumber seedlings in soil infested with Fusarium oxysporum f. sp. cucumerinum KW2-1 (FOC) at 3% (w/w) was zero at the 30th day after sowing when C. rosea 67-1 was applied at 10⁶ spores/g soil following soil fumigation with Dazomet at 1 g/kg of soil, while those were 13.3% and 44.5% in soil treated with Dazomet and 67-1 alone, respectively. Experiments in an infested sunlight greenhouse demonstrated that soil fumigation with Dazomet at 25 g/m² in combination with hole application of C. rosea at 10⁷ spores/hole when transplanting led to 98.5% of control efficiency, much higher than those resulted from treatments of fumigation (88.8%) and biocontrol (62.2%). Cucumber yield was increased by 48.5% by combinative treatment. Analysis of Bliss Independence indicated that there was a synergistic effect of Dazomet and C. rosea 67-1 in combating cucumber fusarium wilt. Tests with soil dilution plates showed that population of FOC declined drastically and remained at a low level during the whole growing season and the colonization of 67-1 in rhizosphere enhanced greatly (P<0.01) after chemical fumigation followed by application of the 67-1. The research indicated that combination of fumigation and biocontrol could protect cucumber from infection by FOC and might also be used for management of other soil born diseases.