Identification and Pathogenicity of Entomopathogenic Fungi BB-T02 against Two Thrip Species

doi:10.16409/j.cnki.2095-039x.2020.06.009

Abstract

Abstract: Gynaikothrips uzeli and Thrips hawaiiensis are serious thrip pests belonging to Phlaeothripidae and Thripidae of Thysanoptera, respectively. At present, the biocontrol fungus resources of these two thrip species are few. In this study, some biocontrol fungi were isolated from fungus-infected insects, and their pathogenicity and taxonomic position were determined. One strain of the entomopathogenic fungus, BB-T02, was isolated from the Dendrolimus punctatus larvae that died naturally of fungal infection. The strain BB-T02 was identified as Beauveria bassiana on the basis of morphological observations and molecular analysis. Against G. uzeli and T. hawaiiensis second instar nymphs, the LC₅₀ values of the strain BB-T02 were 1.89×10⁵ spores/mL and 9.90×10⁴ spores/mL at 7 d after infection, the cumulative-corrected mortalities were 94.12% and 98.81%, and the LT₅₀ values were 3.48 d and 3.14 d for the 10⁸ spores/mL treatment, respectively. Against G. uzeli and T. hawaiiensis adults, the LC₅₀ values of the strain BB-T02 were 4.45×10⁵ spores/mL and 2.13×10⁵ spores/mL at 8 d after infection, the cumulative-corrected mortalities were 87.21% and 95.29%, and the LT₅₀ values were 4.20 d and 3.69 d for the 10⁸ spores/mL treatment, respectively. In conclusion, with strong pathogenicity against G. uzeli and T. hawaiiensis, the B. bassiana BB-T02 shows an excellent biocontrol potential and promising application prospect.

Key words: Beauveria bassiana, isolation and identification, Gynaikothrips uzeli, Thrips hawaiiensis, pathogenicity

CLC Number:

S433
S476.1

HUANG Peng, YAO Jinai, YU Deyi, HOU Xiangyu. Identification and Pathogenicity of Entomopathogenic Fungi BB-T02 against Two Thrip Species[J]. Chinese Journal of Biological Control, 2020, 36(6): 929-937.

References

[1] 福建省人民政府. 福建省人民政府关于加快农业七大优势特色产业发展的意见[DB/OL]. (2017-08-23)(2019-05-31). http://www.fujian.gov.cn/zc/zxwj/szfwj/201708/t20170823_1477225.htm.
[2] 于伟国. 发展特色现代农业加快乡村振兴步伐[J]. 理论参考, 2018(4):22-23.
[3] 余德亿, 黄鹏, 姚锦爱, 等. 盆栽榕树蓟马种类及优势种榕管蓟马对寄主植物的致害性[J]. 昆虫学报, 2012, 55(7):832-840.
[4] 福建省统计局. 福建统计年鉴-2019. (2019-03-04)(2019-11-25). http://tjj.fujian.gov.cn/tongjinianjian/dz2019/index.htm.
[5] 余德亿, 姚锦爱, 黄鹏, 等. 榕管蓟马危害与寄主叶片结构及营养物质的关系[J]. 南京农业大学学报, 2014, 37(2):38-44.
[6] Yu D Y, Huang P, Chen Y, et al. Effects of flower thrips (Thysanoptera:Thripidae) on nutritional quality of banana (Zingiberales:Musaceae) buds[J]. PLoS ONE, 2018, 13(8):e0202199.
[7] 毛加梅, 岳建强, 高俊燕. 我国果树蓟马研究进展[J]. 江西农业学报, 2013, 25(12):69-74.
[8] 付步礼, 曾东强, 刘奎, 等. 蓟马类害虫抗药性研究进展[J]. 农学学报, 2014, 4(3):28-34.
[9] 韩云, 唐良德, 吴建辉. 蓟马类害虫综合治理研究进展[J]. 中国农学通报, 2015, 31(22):163-174.
[10] Shah P A, Pell J K. Entomopathogenic fungi as biological control agents[J]. Applied Microbiology and Biotechnolog, 2003, 61(5-6):413-423.
[11] 王利军, 谭万忠, 罗华东, 等. 虫生真菌及其在害虫生物控制中的应用现状与展望[J]. 河南农业科学, 2010, 39(4):119-125.
[12] 宋晓兵, 彭埃天, 程保平, 等. 一株侵染柑橘木虱的球孢白僵菌的分离及鉴定[J]. 植物保护, 2017, 43(4):139-144.
[13] 余德亿, 黄鹏, 姚锦爱, 等. 蜡蚧轮枝菌V3450菌株对榕管蓟马及斯氏钝绥螨的毒力比较[J]. 西北农林科技大学学报(自然科学版), 2015, 43(8):133-139.
[14] 曾丽琼, 何学友, 蔡守平, 等. 白僵菌和绿僵菌感染榕管蓟马成虫试验[J]. 防护林科技, 2019(2):8-10.
[15] Makoto A, Takafumi I. Susceptibility of five species of thrips to different strains of the entomopathogenic fungus, Beauveria bassiana[J]. Applied Entomology and Zoology, 2005, 40(4):667-674.
[16] 谢小聪. 黄胸蓟马SCAR分子标记及其生防真菌的初步研究[D]. 福州:福建农林大学, 2013, 25-28.
[17] Wassermann M, Selzer P, Steidle J L M, et al. Biological control of Ixodes ricinus larvae and nymphs with Metarhizium anisopliae blastospores[J]. Ticks Tick-borne Diseases, 2016, 7(5):768-771.
[18] Wang J J, Bai W W, Zhou W, et al. Transcriptomic analysis of two Beauveria bassiana strains grown on cuticle extracts of the silkworm uncovers their different metabolic response at early infection stage[J]. Journal of Invertebrate Pathology, 2017, 145:45-54.
[19] Lu L M, Cheng B P, Du D C, et al. Morphological, molecular and virulence characterization of three Lencanicillium species infecting Asian citrus psyllids in Huangyan citrus groves[J]. Journal of Invertebrate Pathology, 2015, 125:45-55.
[20] 王俊平, 郑长英. 对蓟马类害虫高致病性球孢白僵菌的分离、鉴定[J]. 茶叶科学, 2011, 31(4):295-299.
[21] 蒲蛰龙, 李增智. 昆虫真菌学[M]. 合肥:安徽科学技术出版社, 1996.
[22] 侯颖, 夏彦飞, 徐建强, 等. 一株绿僵菌的鉴定、生物学特性及对东亚飞蝗的致病力[J]. 中国生物防治学报, 2015, 31(3):333-339.
[23] 邹东霞, 史国英, 廖旺姣, 等. 桉蝙蛾高毒力白僵菌菌株筛选及鉴定[J]. 中国森林病虫, 2016, 35(5):1-4.
[24] 刘振邦. 苏云金芽孢杆菌晶体杀虫蛋白Cry1Ac基因在虫生真菌球孢白僵菌中的表达[D]. 合肥:安徽农业大学, 2011, 1.
[25] 何恒果, 李正跃, 陈斌, 等. 虫生真菌对害虫防治的研究与应用[J]. 云南农业大学学报, 2004, 19(2):167-173.
[26] 汪黎明. 几株球孢白僵菌基因工程菌的遗传稳定性[D]. 合肥:安徽农业大学, 2011.
[27] 葛文超, 杜广祖, 赵永鑫, 等. 西花蓟马高毒力虫生真菌筛选及其生防应用潜力的研究进展[J]. 生物安全学报, 2018, 27(4):240-248.
[28] 袁盛勇, 张宏瑞, 孔琼, 等. 球孢白僵菌MZ060812菌株对西花蓟马的致病性研究[J]. 西北农林科技大学学报(自然科学版), 2010, 38(3):145-149
[29] 袁盛勇, 张宏瑞, 孔琼, 等. 球孢白僵菌MZ041016菌株对西花蓟马的毒力测定[J]. 华中农业大学学报, 2011, 30(2):197-199.
[30] 袁盛勇, 孔琼, 薛春丽, 等. 球孢白僵菌对棕榈蓟马的毒力测定[J]. 中国蔬菜, 2013(16):92-95.
[31] 刘晓晨, 吴圣勇, 雷仲仁, 等. 不同温度下两株球孢白僵菌侵染西花蓟马的生长动力学及其毒力[J]. 中国农业科学, 2018, 51(8):1484-1492.
[32] 李银平, 雷仲仁, 王海鸿, 等. 对西花蓟马高效的球孢白僵菌菌株筛选及产孢特性研究[J]. 中国生物防治学报, 2013, 29(2):219-226.
[33] 张建伟, 王中康, 申剑飞, 等. 小菜蛾高致病力绿僵菌的筛选、鉴定及培养特性研究[J]. 中国生物防治学报, 2012, 28(1):53-61.
[34] 王静, 雷仲仁, 徐洪富, 等. 白僵菌对西花蓟马若虫的致病力和对巴氏钝绥螨的影响[J]. 中国生物防治学报, 2011, 27(4):479-484.
[35] 黄鹏, 姚锦爱, 余德亿. 金龟子绿僵菌FM−03生物学特性及其对柑橘粉蚧的侵染[J]. 中国生物防治学报, 2018, 34(6):858-865.