GFP labeling of Bacillus paralicheniformis ZYGT1811 and Its Colonization Dynamics in Wheat Plants and Rhizosphere Soil

doi:10.16409/j.cnki.2095-039x.2025.02.069

Abstract

Abstract: This study employed electroporation to generate the ZYGT1811-GFP labeled strain exhibiting green fluorescence, aiming to elucidate regulatory mechanism of Bacillus paralicheniformis ZYGT1811 on wheat crown rot. Assessment of growth curve, antagonistic activity, motility, and biofilm formation revealed that the incorporation of foreign genes did not significantly impact the original strain. Colonization of strain ZYGT1811-GFP in wheat plants and rhizosphere soil was elucidated using root irrigation. The findings indicated that the colonization of ZYGT1811-GFP in the wheat rhizosphere soil, roots, stems, and leaves peaked at 6 days after root irrigation, with population densities 3.24× 10⁶, 1.76× 10⁶, 9.00× 10⁵, and 6.47× 10⁴ cfu/g, respectively. Colonization diminished on the 21st day, although persisted at a certain level, with population density ranking as follows: rhizosphere soil>root>stem>leave. The above results indicate that ZYGT1811 possesses sustainable colonization capability, which offers a theoretical foundation for elucidating the control mechanism of Bacillus paralicheniformis against wheat crown rot.

Key words: wheat crown rot, green fluorescent protein (GFP), biocontrol, Bacillus paralicheniformis, colonization

CLC Number:

S476

WANG Yixuan, LIU Jinglong, LI Huixia, FEI Shaodan, SUN Jiacong, REN Xingping, TIAN Xiaoming, LIU Yonggang, ZHANG Haiying. GFP labeling of Bacillus paralicheniformis ZYGT1811 and Its Colonization Dynamics in Wheat Plants and Rhizosphere Soil[J]. Chinese Journal of Biological Control, 2025, 41(6): 1403-1411.

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