Control Effect of Combined Bacillus Species on Fusarium tricinctum Leaf Spot Disease of Hemerocallis citrine

doi:10.16409/j.cnki.2095-039x.2026.02.005

Abstract

Abstract: Leaf spot disease is one of the most serious diseases of Hemerocallis citrine, and the main pathogen of the disease is Fusarium tricinctum in Shanxi province. In order to control the disease more effectively, the individual effect and allied effect of Bacillus velezensis TG4 and Bacillus amyloliquefaciens YP4 were investigated by pot experiments plant using spray inoculation, and the biocontrol mechanism of two bacteria was preliminarily explored. The result showed that both two bacteria had protective and therapeutic effects. The pellet, fermentation supernatant and volatile substances of TG4 and YP4 had good antibacterial activity against Fusarium tricinctum KHT2. The antagonistic synergistic ratio of 10⁸ cfu/mL bacterial suspension mixed with two bacteria in equal volume (5:5) was 1.03, and the synergistic ratio of pot control effect was 1.05, indicating that the treatment had mixed synergistic effect. The synergistic ratios of antagonistic effect and pot control effect of 10⁷ cfu/mL bacterial suspension mixed with two bacteria in equal volume (5:5) were 9.67 and 9.97, respectively. It could be used as a ratio scheme for the production of two biocontrol bacteria mixed bacterial solution. The results can provide data support for the prevention of F. tricinctum leaf spot disease of Hemerocallis citrine and the study on the combined use of biocontrol bacteria.

Key words: leaf spot of Hemerocallis citrine, Fusarium tricinctum, Bacillus velezensis, Bacillus amyloliquefaciens, combined use of biocontrol bacteria

CLC Number:

S476

ZHOU Jianbo, MENG Qingge, ZHAO Tong, ZHAO Yan, CAO Yuru, KONG Kaishui, LI Jie. Control Effect of Combined Bacillus Species on Fusarium tricinctum Leaf Spot Disease of Hemerocallis citrine[J]. Chinese Journal of Biological Control, 2026, 42(1): 64-71.

References

[1] Wang H, Zhao M, Wu Z Z, et al. Nutrient composition and functional constituents of daylily from different producing areas based on widely targeted metabolomics[J]. Food Chemistry: X, 2024, 21: 101239.
[2] Jiang C, Zhang W, Zhang Y, et al. Integrated metabolomic and transcriptomic analysis revealed the transition of functional components in edible flower buds of Hemerocallis citrina Baroni[J]. Food Chemistry: X, 2024, 24: 101852.
[3] 孙瑞卿, 王宁, 秦曙, 等. 黄花菜病虫害防治现状、问题和对策[J]. 中国植保导刊, 2023, 43(6): 52-55.
[4] 孟庆格. 黄花菜叶斑病病原群落结构分析及其生物防治研究[D]. 太谷: 山西农业大学, 2025.
[5] 颜宇科, 赵吉桃, 何静, 等. 裂褶菌HJ-18发酵滤液对三线镰刀菌的抑菌机理研究[J]. 植物保护, 2025, 51(1): 153-160, 189.
[6] 王书翰, 谢学文, 李磊, 等. 解淀粉芽胞杆菌YB114对黄瓜棒孢叶斑病的生防效果及发酵条件优化[J]. 植物保护, 2023, 49(6): 131-138.
[7] 马佳, 李颖, 胡栋, 等. 芽胞杆菌生物防治作用机理与应用研究进展[J]. 中国生物防治学报, 2018, 34(4): 639-648.
[8] Liu Y, Tian Y, Yue L, et al. Effectively controlling Fusarium root rot disease of Angelica sinensis and enhancing soil fertility with a novel attapulgite-coated biocontrol agent[J]. Applied Soil Ecology, 2021, 168: 104121.
[9] 韦吉霞, 何元胜, 郑元仙, 等. 雪茄烟枯萎病茄病镰刀菌生物学特性、拮抗细菌筛选和药剂毒力测定[J/OL]. 分子植物育种, 2024. https://link.cnki.net/urlid/46.1068.S.20240926.1108.002.
[10] 祝文慧, 秦溥, 赵岩, 等. 两种芽胞杆菌对苗期土传辣椒疫病的防控作用[J]. 中国生物防治学报, 2023, 39(5): 1235-1243.
[11] 邓安玲, 常若葵, 李卓然, 等. 贝莱斯芽孢杆菌挥发性有机化合物对番茄灰葡萄孢的抑制及活性组分分析[J]. 植物病理学报, 2025, 55(2): 301-312.
[12] 张文静, 徐大勇, 吴倩琳, 等. 拮抗番茄灰霉病的贝莱斯芽孢杆菌XDY66基因组分析[J]. 园艺学报, 2024, 51(6): 1413-1425.
[13] 毕钰, 张琳, 余红凤, 等. 小麦赤霉病拮抗菌JB7的拮抗活性及鉴定[J]. 微生物学报, 2024, 64(5): 1580-1592.
[14] 谢林艳, 何丽莲, 罗艳菊, 等. 生防菌YC89在不同甘蔗品种中定殖及防治赤腐病的研究[J]. 中国生物防治学报, 2024, 40(5):1066-1073.
[15] 黄大野, 曹春霞, 张亚妮, 等. 枯草芽孢杆菌NBF809防治番茄棒孢叶斑病研究[J]. 中国蔬菜, 2018(12): 40-44.
[16] Uwaremwe C, Yue L, Wang Y, et al. An endophytic strain of Bacillus amyloliquefaciens suppresses Fusarium oxysporum infection of Chinese wolfberry by altering its rhizosphere bacterial community[J]. Frontiers in Microbiology, 2022, 12: 782523.
[17] 柴庆凯, 张斌, 常若葵, 等. 解淀粉芽孢杆菌LJ02对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究[J]. 植物病理学报, 2019, 49(6): 828-835.
[18] 张德锋, 高艳侠, 王亚军, 等. 贝莱斯芽孢杆菌的分类、拮抗功能及其应用研究进展[J]. 微生物学通报, 2020, 47(11): 3634-3649.
[19] 吴慧芳, 蒙耀, 石晖琴, 等. 一株马铃薯干腐病生防菌的鉴定及其抑菌活性研究[J]. 植物保护, 2024, 50(3): 111-120.
[20] Kosman E, Cohen Y. Procedures for calculating and differentiating synergism and antagonism in action of fungicide mixtures[J]. Phytopathology, 1996， 86(11): 1263-1272.
[21] 张嘉城, 方香玲, 南志标. 植物病原镰刀菌产生的毒素种类及其危害[J]. 草业科学, 2021, 38(8): 1513-1524.
[22] Kosiak B, Torp M, Skjerve E, et al. The prevalence and distribution of Fusarium species in Norwegian cereals: a survey[J]. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science, 2003, 53(4): 168-176
[23] Shi W, Tan Y, Wang S, et al. Mycotoxigenic potentials of Fusarium species in various culture matrices revealed by mycotoxin profiling[J]. Toxins, 2016, 9(1): 6.
[24] Wiśniewska H, Stępień Ł, Waśkiewicz A, et al. Toxigenic Fusarium species infecting wheat heads in Poland[J]. Central European Journal of Biology, 2014, 9: 163-72.
[25] 彭成彬, 柯栋贤, 陈美霞, 等. 茶树镰刀菌的分离鉴定和遗传多样性研究[J]. 中国茶叶, 2018, 40(8): 24-29.
[26] 牟晓玲, 李潇潇, 师桂英, 等. 兰州百合枯萎病病原菌鉴定及罹病组织超微结构观察[J]. 植物保护学报, 2022, 49(4): 1111-1118.
[27] 廖承树, 罗晓锋, 岳滨, 等. 两株生防菌对华重楼主要病害的防效试验[J]. 南方农业, 2023, 17(5): 113-119.
[28] 沈艳, 祝友朋. 两株生防菌对蓝莓根腐病菌的抑制作用[J]. 北方果树, 2024(5): 21-23.