Screening of Bioactive Volatiles from Matthiola incana against Colletotrichum gloeosporioides and Preliminary Investigation into Their Action Mechanisms

doi:10.16409/j.cnki.2095-039x.2026.02.025

Abstract

Abstract: To elucidate inhibitory effects of hoary stock (Matthiola incana) volatiles on grape anthracnose pathogen Colletotrichum gloeosporioides and to identify key bioactive components, mycelial growth rate method, spore germination assay, and detached fruit inoculation method were employed to systematically evaluate the effects of hoary stock mixed volatiles at varying concentrations, as well as individual volatile monomers, on colony growth, spore germination, and pathogenicity of C. gloeosporioides. The antimicrobial mechanism was preliminarily investigated by measuring changes in mycelial extracellular conductivity and key enzyme activities. The results demonstrated that hoary stock mixed volatiles exerted significant dose-dependent inhibitory effects on C. gloeosporioides, and the combined leaf-flower volatiles exhibited superior antimicrobial efficacy compared to leaf or flower volatiles alone. From a panel of 37 volatile monomers, the most potent antimicrobial compounds within the phenol, alcohol, ester, terpene, ketone, aldehyde, and alkane classes were identified as eugenol, 1-octen-3-ol, ethyl benzoate, 1,8-cineole, 6-methyl-5-hepten-2-one, 1,1-diethoxy-ethane, and tridecane, with EC₅₀ values for mycelial growth inhibition of 22.54、 92.80、 124.80、 220.83、 293.85、 1.29×10³ and 1.55×10⁴ μL/L, respectively. All seven monomers significantly suppressed spore germination (P＜0.05), with 1-octen-3-ol and 6-methyl-5- hepten-2-one demonstrating the most pronounced inhibitory effects. Following treatment with the seven monomers, mycelial extracellular conductivity was significantly elevated, while the activities of CAT, POD, SOD, NAD-MDH, and SDH were reduced, suggesting that the antifungal mechanism may involve disruption of cell membrane integrity, interference with the antioxidant defense system, and inhibition of energy metabolism. The bioactive volatile monomers identified from hoary stock in this study demonstrated considerable potential for development as plant-derived green fungicides or postharvest fumigants.

Key words: Matthiola incana, grape anthracnose, volatile organic compound, antimicrobial activity

CLC Number:

S476

SONG Wenwen, JING Yuanjing, YANG Rongliang, WANG Huimin, WANG Yuan, DU Fei, LI Huiming, DENG Weiping. Screening of Bioactive Volatiles from Matthiola incana against Colletotrichum gloeosporioides and Preliminary Investigation into Their Action Mechanisms[J]. Chinese Journal of Biological Control, 2026, 42(3): 738-749.

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