Isolation, Identification of Active Compounds Derived from Streptomyces HBERC-44441, and Bioassay of Their Antimicrobial Activity

doi:10.16409/j.cnki.2095-039x.2023.02.027

Abstract

Abstract: Streptomyces sp. HBERC-44441 was isolated from the soil sample collected from Jougongshan Mountain, Tongshan County, Hubei Province. Based on scanning electron microscopic (SEM) observation and 16S rRNA gene sequence analysis, the strain was identified as S. lydicus HBERC-44441.The bioassay results showed that the fermentation extracts of the strain exhibited excellent fungicidal activities against some important crop pathogenic fungi such as Rhizoctonia solani, Fusarium culmorum, Botrytis cinerea and so on. Several active compounds were purified by preparative HPLC, and the chemical structures of four compounds were elucidated by nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HRMS), they are indol-3-carboxylic acid, pimprinine, and two novel pimprinine dimers, respectively. The antimicrobial activity of pure compounds showed that pimprinine and its dimer had weak activity against some pathogenic fungi, while indol-3-carboxylic acid showed moderate activity. We found that pimprinine had moderate activity against important crop bacterial wilt for the first time, the MIC value is 25 µg/mL.

Key words: Streptomyces lydicus, pimprinine, indol-3-carboxylic acid, compounds, antimicrobial activity

CLC Number:

S476

WAN Zhongyi, FANG Wei, ZHANG Zhigang, WU Zhaoyuan, LI Taiming, WANG Kaimei, YANG Ziwen. Isolation, Identification of Active Compounds Derived from Streptomyces HBERC-44441, and Bioassay of Their Antimicrobial Activity[J]. Chinese Journal of Biological Control, 2023, 39(3): 615-623.

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