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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (3): 626-635.DOI: 10.16409/j.cnki.2095-039x.2022.02.025

• RESEARCH REPORTS • Previous Articles    

Function Analysis of a HutC Gene of the GntR Family in Lysobacter capsici X2-3

LI Zhiyuan, ZHAO Dan, HAN Shengnan, HAN Chao, LIU Aixin   

  1. College of Plant Protection, Shandong Agricultural University, Taian 271018, China
  • Received:2022-01-10 Published:2022-06-20

Abstract: GntR is a large family of transcriptional regulators and is further divided into seven subfamily based on the sequence similarities of C-terminal effector binding. As the second-largest subfamily of GntR, HutC comprise about 30% of GntR regulators. HutC has been reported in regulating various biological processes in divers of bacteria, however little is known about its regulation in PGPR(plant growth-promoting rhizobacteria) especially in Lysobacter capsici. The goal of this study was to analyze the functional characteristic of a HutC gene in Lysobacter capsici. As showed in this study, deletion of LC_HutC in L. capsici X2-3 reduced the bacterial growth rate, motility, chemotaxis to wheat root exudates and rhizosphere colonization of wheat, and increased the biofilm formation significantly. However, no significant changes in the antimicrobial activity of the LC_HutC mutant against either fungi or oomycetes were observed. These results suggested the possible role of LC_HutC in regulating chemotaxis, rhizosphere colonization and cell motility in L.capsici X2-3. This work expands our understanding of HutC in regulation of chemotaxis and colonization in PGPR of L. capsici.

Key words: Lysobacter capsici, HutC transcription factor, biofilm, colonization, antimicrobial activity

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