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journal1 ›› 2015, Vol. 31 ›› Issue (6): 868-875.DOI: 10.16409/j.cnki.2095-039x.2015.06.008

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Isolation and Identification of an Entomopathogenic Fungal Strain and Its Virulence to Larvae of Melanotus cribricollis

ZHANG Yabo, YE Bihuan, WU Xiaoshuang, WANG Haojie, SHU Jinping   

  1. Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China
  • Received:2015-08-11 Online:2015-12-08 Published:2015-12-17

Abstract: Metarhizium spp. is an important entomopathogenic fungi, which have been using world widely to control agricultural insect pests. A strain of entomopathogenic fungi isolated from an infected larva of Popillia sp. was identified as M. flavoviride var. pemphigi by both morphological and DNA sequencing of 5' end of EF- of the isolate. The results of optimization of culture condition showed that lactose benefited the growth of mycelium, while as glucose was conducive to the conidia production. Its optimum nitrogen source was KNO3. Based on a combination of lactose and KNO3 used as carbon and nitrogen sources, a C/N ratio at 34:1 was the most optimal for mycelium growth and conidia production. Mn as an additive promoted conidia production. A bioassay of M. flavoviride var. pemphigi against Melanotus cribricollis larvae (120-150 mg per capita) was conducted in small cups filled with 50 g soil containing 5×106 conidia per gram of soil. A corrected mortality of M. cribricollis reached 57.14% during a 30-day period under controlled conditions, with a larval escape ratio of 4.76%.

Key words: Metarhizium flavoviride, gene EF-1α, Melanotus cribricollis, biological control

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