Identification of Marine Bacterium BA-3 and Its Control Fusarium oxysporum of Cymbidium ensifolium Stem Rot

doi:10.16409/j.cnki.2095-039x.2019.06.020

Abstract

Abstract: In order to screen the bacterium with good antibacterial effect against Fusarium oxysporum of Cymbidium ensifolium stem rot. The antibacterial activity of the isolated strains was determined to screen the strains with the highest inhibition rate from marine fish. Base on that the screened strains were classified and identified, and the inhibition spectrum and control efficiency were evaluated. The results showed that among isolated the 43 marine bacteria, 10 strains showed significant inhibitory effects on F. oxysporum, with their inhibition zone diameter ranging from 0.60 to 1.60 cm. The strain BA-3 had the strongest inhibition effects:87.69% in the culture medium. Based on morphological characteristics, biochemical characteristics, and molecular biological, BA-3 was identified as Bacillus amyloliquefaciens, which it can inhibit a broad spectrum of phytopathogenic bacteria. Treatment of F. oxysporum of Cymbidium ensifolium Stem rot and BA-3 fermentation solution resulted in 79.05% control efficacy in the lab, and the efficacy of BA-3 fermentation solution against F. oxysporum of Cymbidium ensifolium Stem rot was 74.71%-77.09% in the field, that it is good application.

Key words: marine bacterium, Bacillus amyloliquefaciens, stem rot on Cymbidium ensifolium, Fusarium oxysporum, control

CLC Number:

YAO Jinai, HUANG Peng, CAI Hongjiao, Hou Xiangyu, YU Deyi. Identification of Marine Bacterium BA-3 and Its Control Fusarium oxysporum of Cymbidium ensifolium Stem Rot[J]. Chinese Journal Of Biological Control, 2019, 35(6): 922-929.

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