Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae (Fabricius)

doi:10.16409/j.cnki.2095-039x.2020.02.018

Abstract

Abstract: The cDNA sequences of voltage-gated sodium channel gene of Sitobion avenae were cloned and its typical characteristics were identified, which laid a foundation for further studies of the molecular mechanism of pesticide resistance in S. avenae. The sequences were cloned by RT-PCR and analyzed by DNASTAR Lasergene 7.10 software. Two cDNA sequences were obtained, SaNav1 and SaNav2, with GenBank accession numbers of MN176137 and MN161584, respecitvely. The SaNav1 sequence was 3423 bp, encoding a total of 1141 amino acids. The SaNav2 sequence was 2874 bp, encoding a total of 958 amino acids. The homology alignment found that the similarity of SaNav1 and the part I sodium channel genes of Rhodolospi maidis (XP_026806613) and Myzus persicae (XP_022174241) were as high as 97.81% and 97.91%; the similarity of SaNav2 and the part II sodium channel genes of Rhodolospi maidis (ALB75309.1) and Myzus persicae (XP_022174237.1) were as high as 97.90% and 96.43%, respectively. The cloned sequences contain four homologous domains, each with six transmembrane segments (S1—S6) and a sodium channel-selective key residue "DENS". The sodium channel genes cloned in S. avenae will provide a basis for studying the role of sodium channel in resistance mechanism in S. avenae.

Key words: Sitobion avenae, sodium channel, gene cloning, knockdown resistance

CLC Number:

S476

DUAN Wenbo, WANG Hao, LI Fen, YIN Xinming, WU Shaoying. Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae (Fabricius)[J]. Chinese Journal Of Biological Control, 2020, 36(2): 307-314.

References

[1] Irwin B, Levitan, Leonard K, et al. The Neuron[M]. New York:Oxford UniversityPress, 2002.
[2] Dong K. Insect sodium channels and insecticide resistance. Invertebrate Neuroscience[J]. 2007, 7(1):17-30.
[3] Thackeray J R, Ganetzky B. Developmentally regulated alternative splicing generates a complex array of Drosophila para sodium channel isoforms[J]. The Journal of Neuroscience, 1994, 14(5):2569-2578.
[4] Song W, Liu Z, Tan J, et al. RNA editing generates tissue-specific sodium channels with distinct gating properties[J]. The Journal of Biological Chemistry, 2004, 279(31):32554-32561.
[5] Cestèle S, Catterall W A. Molecular mechanisms of neurotoxin action on voltage-gated sodium channels[J]. Biochimie, 2000, 82(9-10):883-892.
[6] Narahashi T. Neuroreceptors and ion channels as the basis for drug action:past, present, and future[J]. Journal of Pharmacology & Experimental Therapeutics, 2000, 294(1):1-26.
[7] Salgado A J, Coutinho O P, Reis R L, et al. In vivo response to starch-based scaffolds designed for bone tissue engineering applications[J]. Journal of Biomedical Materials Research, 2007, 80(4):983-989.
[8] Silver K S, Song W, Nomura Y, et al. Mechanism of action of sodium channel blocker insecticides (SCBIs) on insect sodium channels[J]. Pesticide Biochemistry and Physiology, 2010, 97(2):87-92.
[9] Dong K, Du Y, Rinkevich F, et al. Molecular biology of insect sodium channels and pyrethroid resistance[J]. Insect Biochemistry and Molecular Biology, 2014, 50(1):1-17.
[10] Silva A P B, Santos J M M, Martins A J. Mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids-a review[J]. Parasites & Vectors, 2014, 7(1):450.
[11] Yang S, Fitches E, Pyati P, et al. Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae)[J]. Pest Management Science, 2015, 71(7):951-956.
[12] 李新. 拟除虫菊酯类杀虫剂研发及市场概况[J]. 农化市场十日讯, 2016(27):17-19.
[13] 陈巨莲. 小麦蚜虫及其防治[M]. 北京:金盾出版社, 2014.
[14] 马春森, 马罡, 赵飞. 气候变暖对麦蚜的影响[J]. 应用昆虫学报, 2014, 51(6):1435-1443.
[15] 宋修超, 郑方强, 戴凤宾, 等. 以麦二叉蚜和麦长管蚜为寄主的烟蚜茧蜂生理生化特性比较研究[J]. 中国生物防治学报, 2012, 28(4):453-459.
[16] 曹雅忠, 尹姣, 李克斌, 等. 小麦蚜虫不断猖獗原因及控制对策的探讨[J]. 植物保护, 2006, 32(5):72-75.
[17] Williamson M S, Denholm L, Bell C A, et al. Knockdown resistance (kdr) to DDT and pyrethroid insecticides maps to a sodium channel gene locus in the housefly (Musca domestica)[J]. Molecular and General Genetics, 1993, 240(1):17-22.
[18] Jiang X Z, Pei Y X, Lei W, et al. Characterization of an insect heterodimeric voltage-gated sodium channel with unique alternative splicing mode[J]. Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology, 2017, 203:149-158.
[19] Zuo Y, Peng X, Wang K, et al. Expression patterns, mutation detection and RNA interference of Rhopalosiphum padi voltage-gated sodium channel genes[J]. Scientific Reports, 2016, 6:30166.
[20] Soderlund D M. Pyrethroids, knockdown resistance and sodium channels[J]. Pest Management Science, 2008, 64(6):610-616.
[21] Vais H, Atkinson S, Pluteanu F, et al. Mutations of the para sodium channel of Drosophila melanogaster identify putative binding sites for pyrethroids[J]. Molecular Pharmacology, 2003, 64(4):914-922.
[22] Vais H, Williamson M S, Goodson S J, et al. Activation of Drosophila sodium channels promotes modification by deltamethrin:Reductions in affinity caused by knock-down resistance mutations[J]. The Journal of General Physiology, 2000, 115(3):305-318.
[23] Foster S P, Paul, Verity L, et al. A mutation (L1014F) in the voltage-gated sodium channel of the grain aphid, Sitobion avenae, is associated with resistance to pyrethroid insecticides[J]. Pest Management Science, 2014, 70(8):1249-1253.
[24] Panini M, Anaclerio, Matteo, et al. Presence and impact of allelic variations of two alternative s-kdr mutations, M918T and M918L, in the voltage-gated sodium channel of the green peach aphid Myzus persicae[J]. Pest Management Science, 2015, 71(6):878-884.
[25] Chen X, Tie M, Chen A, et al. Pyrethroid resistance associated with M918 L mutation and detoxifying metabolism in Aphis gossypii from Bt cotton growing regions of China[J]. Pest Management Science, 2017, 73(11):2353-2359.
[26] Tan J, Liu Z, Nomura Y, Goldin AL, et al. Alternative splicing of an insect sodium channel gene generates pharmacologically distinct sodium channels[J]. Journal of Neuroscience, 2002, 22(13):5300-5309.
[27] 陈梦丽. 基于钠离子通道突变体的拟除虫菊酯杀虫剂抗性的机理研究[D]. 杭州:浙江大学, 2017.
[28] Soderlund D M, Knipple D C. The molecular biology of knockdown resistance to pyrethroid insecticides[J]. Insect Biochemistry and Molecular Biology, 2003, 33(6):563-577.