The Effect and Mechanism of Fennel Crop Rotation on Soil Bacterial Community to Alleviate Replant Failure of Panax notoginseng

doi:10.16409/j.cnki.2095-039x.2020.06.004

Abstract

Abstract: Panax notoginseng is a characteristic Chinese medicinal plant, but its cultivation was severely hampered by replant failure. Crop rotation is an effective measure to alleviate this problem. In this study, fennel was cultivated as the succeeding crop of P. notoginseng, and the rhizosphere soil of fennel was collected to make soil extract to assess the effect of fennel rotation on seedling survival rate and soil bacterial community of P. notoginseng. The seedlings survival rate was significantly increased by irrigating soil water extract from fennel rotation soil. In addition, the soil bacterial community was significantly changed with the increasing of richness index. At phylum level, the dominant phylum with relative abundance >1%, Proteobacteria and Actinobacteria, were significantly enriched. At genus level, the relative abundances of 66 genera were significantly increased and 30 genera were decreased. Among them, there are 14 dominant genera (the relative abundance was more than 0.2%) with significant increase and 8 dominant genera with significant decrease. In general, the number of increasing genera was more than that of decreasing genera. The dominant genera with significantly increased include the common antagonistic bacteria Pseudomonas and Bacillus. One strain of Bacillus and two strains of Pseudomonas isolated from the rhizosphere soil of fennel exhibited strong antagonistic activity to root rot pathogens Fusarium solani and Monographella cucumerina. In general, crop rotation of fennel can increase the seedlings survival rate of P. notoginseng and enrich the abundance of antagonism bacteria in bacterial community. It is potential to alleviate replant failure of P. notoginseng by rotation of fennel and P. notoginseng.

Key words: Panax notoginseng, fennel, crop rotation, seedlings survival rate, bacterial community, Bacillus, Pseudomonas

CLC Number:

S432.1

LIU Haijiao, SU Yingwei, FANG Lan, LUO Lifen, WANG Luotao, ZHANG Zilong, ZHU Shusheng, YANG Min. The Effect and Mechanism of Fennel Crop Rotation on Soil Bacterial Community to Alleviate Replant Failure of Panax notoginseng[J]. Chinese Journal of Biological Control, 2021, 37(1): 139-149.

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