Screening and Identification of Bacillus velezensis Strain BP-1 and the Field Control Efficiency against Peanut Web Blotch

doi:10.16409/j.cnki.2095-039x.2021.02.004

Abstract

Abstract: In order to obtain effective resources for controlling peanut web blotch,60 healthy peanut leaves were collected from major peanut producing areas in Liaoning province, such as Fuxin, Shenyang, Jinzhou, etc. 435 bacteria, 98 fungi and 30 actinomycetes were screened by dilution plate method. 34 strains showed different levels of resistance against Peyronellaea arachidicola. Among these strains, BP-1 strain showed the strongest inhibitory activity, and the inhibition zone was 27 mm. Meanwhile, BP-1 strain showed inhibitory activity against other 11 kinds of pathogens,with the inhibition zone among 5.6 to 22.8 mm. According to the morphological characteristics, physiological and biochemical reaction and 16S rDNA sequence comparative analysis, BP-1 strain was identified as Bacillus velezensis. BP-1 fermentation broth showed good control effect on peanut web blotch, with the efficacy of 60.65% after spraying germination broth diluted 200 times. Therefore, the BP-1 had a great potential in controlling peanut web blotch.

Key words: Peyronellaea arachidicola, biological control, inhibitory action, Bacillus velezensis

CLC Number:

S476

ZANG Chaoqun, ZHAO Ying, XIE Jinhui, LIN Ying, PEI Xue, LIU Xiaozhou, LIANG Chunhao. Screening and Identification of Bacillus velezensis Strain BP-1 and the Field Control Efficiency against Peanut Web Blotch[J]. Chinese Journal of Biological Control, 2021, 37(2): 259-265.

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