Study of Biocontrol Efficacy on Cucurbit Gummy Stem Blight by Lysobacter capsici NF87-2

doi:10.16409/j.cnki.2095-039x.2021.06.023

Abstract

Abstract: Cucurbit gummy stem blight caused by Didymella bryoniae is an important soil-borne fungal diseases and cause significant economic losses. In order to screen and evaluate the antagonistic bacteria against D. bryoniae, the antagonistic activity in vitro and control effect on cucurbit gummy stem blight in pot experiment were investigated in this study. Antagonistic examination against D. bryoniae in vitro revealed that Lysobacter capsici NF87-2 showed strong and steady antagonism, with the inhibition rate of 81.6%. Inhibition rates of strain NF87-2 and its secondary metabolites on spore germination of D. bryoniae were 59.4% and 67.2%, respectively. Seed germination percentage and seedling emergence rate were 83% and 82%, respectively, after inoculated with L. capsici NF87-2 and D. bryoniae together while 48% and 38%, respectively, after only inoculated with D. bryoniae. The fermented liquid and its secondary metabolite of strain NF87-2 could also effectively control gummy stem blight on cucumber in pot experiment, with the control efficiency of 81.6% and 66.5%, respectively. Therefore, strain NF87-2 was a prospective antagonistic bacterium and it is hopeful to be developed as a biological pesticide to control cucurbit gummy stem blight.

Key words: Didymella bryoniae, Lysobacter capsici NF87-2, inhibition rate, control effect

CLC Number:

S476

ZUO Yang, ZHANG Xinning, QIAO Junqing, LI Yuerong, LIU Yongfeng, LIU Youzhou. Study of Biocontrol Efficacy on Cucurbit Gummy Stem Blight by Lysobacter capsici NF87-2[J]. Chinese Journal of Biological Control, 2021, 37(6): 1241-1249.

References

[1] 贺字典,高玉峰,董尊,等.复配杀菌剂筛选及其对黄瓜蔓枯病防治效果测定[J].河北科技师范学院学报, 2019, 33(3):35-39.
[2] 陈开端,韩翠婷,戴峥峰,等.浅谈西甜瓜蔓枯病和枯萎病的诊断与防治[J].中国蔬菜, 2019(8):104-105.
[3] 陈熙,楼兵干,陈恩茂,等.西瓜蔓枯病研究[J].中国蔬菜, 1991(1):1-3.
[4] 刘书林,顾兴芳,石延霞,等.瓜类蔬菜蔓枯病研究概况[J].中国蔬菜, 2013(18):1-10.
[5] 李雨,王少秋,谭蕊,等.西瓜蔓枯病有效药剂筛选及药效评价[J].农药, 2016, 55(6):460-462.
[6] 张旭辉,张红楠,李勇,等.抑制西瓜蔓枯病菌的生防真菌筛选、鉴定及发酵条件优化[J].中国生物工程杂志, 2017, 37(5):76-86.
[7] Martinez B, Perez J, Infante D, et al. Antagonism of Trichoderma spp. isolates against Didymella bryoniae (Fuckel) Rehm. Revista de Protección Vegetal, 2013, 28(3):192-198.
[8] Ruangwong O, Wonglom P, Phoka N, et al. Biological control activity of Trichoderma asperelloides PSU-P1 against gummy stem blight in muskmelon (Cucumismelo)[J]. Physiological and Molecular Plant Pathology, 2021, 115. https://doi.org/10.1016/j.pmpp.2021.101663.
[9] Sudisha J, Niranjana S R, Umesha S, et al. Transmission of seed-borne infection of muskmelon by Didymella bryoniae and effect of seed treatments on disease incidence and fruit yield[J]. Biological Control, 2006, 37(2):196-205.
[10] Kaewkham T, Hynes R K, Siri B. The effect of accelerated seed ageing on cucumber germination following seed treatment with fungicides and microbial biocontrol agents for managing gummy stem blight by Didymella bryoniae[J]. Biocontrol Science and Technology, 2016, 26(8):1048-1061.
[11] Punja Z K, Tirajoh A, Collyer D, et al. Efficacy of Bacillus subtilis strain QST 713(Rhapsody) against four major diseases of greenhouse cucumbers[J]. Crop Protection, 2019, 124:104845.
[12] Nga N, Giau N T, Long N T, et al. Rhizobacterially induced protection of watermelon against Didymella bryoniae[J]. Journal of Applied Microbiology, 2010, 109(2):567-582.
[13] 白如霞,曾汇文,范倩,等.撕裂蜡孔菌对黄瓜蔓枯病的防治作用及促生增产效果[J].中国农业科学, 2019, 52(15):2604-2615.
[14] 刘邮洲,陈夕军,乔俊卿,等.一株萎缩芽胞杆菌YL3的鉴定及其脂肽类化合物分析[J].中国生物防治学报, 2017, 33(1):142-150.
[15] Liu Y Z, Qiao J Q, Liu Y F, et al. Characterization of Lysobacter capsici strain NF87-2 and its biocontrol activities against phytopathogens[J]. European Journal of Plant Pathology, 2019, 155(3):859-869.
[16] Qian G L, Hu B S, Jiang Y H, et al. Identification and characterization of Lysobacter enzymogenes as a biological control agent against some fungal pathogens[J]. Agricultural Sciences in China, 2009, 8(1):68-75.
[17] Zhao Y, Qian G, Chen Y, et al. Transcriptional and antagonistic responses of biocontrol strain Lysobacter enzymogenes OH11 to the plant pathogenic oomycete Pythium aphanidermatum[J]. Frontier in Microbiology, 2017, 8:1025.
[18] Kilic-Ekici O, Yuen G Y. Comparison of strains of Lysobacter enzymogenes and PGPR for induction of resistance against Bipolaris sorokiniana in tall fescue[J]. Biological Control, 2004, 30(2):446-455.
[19] Palumbo J D, Sullivan R F, Kobayashi D Y. Molecular characterization and expression in Escherichia coli of three beta-1,3-glucanase genes from Lysobacter enzymogenes strain N4-7[J]. Journal of Bacteriology, 2003, 185:4362-4370.
[20] Wang Y, Zhao Y, Zhang J, et al. Transcriptomic analysis reveals new regulatory roles of Clp signaling in secondary metabolite biosynthesis and surface motility in Lysobacter enzymogenes OH11[J]. Applied Microbiology and Biotechnology, 2014, 98(21):9009-9020.
[21] Laborda P, Chen X, Wu G, et al. Lysobacter gummosus OH17 induces systemic resistance in Oryza sativa'Nipponbare'[J]. Plant Pathology, 2020, 69:838-848.
[22] Ji G H, Wei L F, He Y Q, et al. Biological control of rice bacterial blight by Lysobacter antibioticus strain 13-1[J]. Biological Control, 2008, 45:288-296.
[23] Ko H S, Jin R D, Krishnan H B, et al. Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes[J]. Current Microbiology, 2009, 59:608-615.
[24] Lee Y S, Naning K W, Nguyen X H, et al. Ovicidal activity of lactic acid produced by Lysobacter capsici YS1215 on eggs of root-knot nematode, Meloidogyne incognita[J]. Journal of Microbiology and Biotechnology, 2014, 24:1510-1515.
[25] Lee Y S, Nguyen X H, Naning K W, et al. Role of lytic enzymes secreted by Lysobacter capsici YS1215in the control of root-knot nematode of tomato plants[J]. Indian Journal of Microbiology, 2015, 55:74-80.
[26] Puopolo G, Cimmino A, Palmieri M C, et al. Lysobactercapsici AZ78 produces cyclo (L-Pro-L-Tyr), a2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola[J]. Journal of Applied Microbiology, 2014, 117:1168-1180.
[27] Puopolo G, Giovannini O, Pertot I. Lysobacter capsici AZ78 can be combined with copper to effectively control Plasmopara viticola on grapevine[J]. Microbiological Research, 2014, 169:633-642.
[28] Santos G, Tschoeke P H, Sarmento R A, et al. Impact of growing seasons and pesticides used on the occurrence and severity of the gummy stem blight in melon cultivation in Brazil[J]. Plant Pathology, 2021, https://doi.org/10.1007/s10658-021-02312-w.
[29] Rennberger G, Turechek W W, Keinath A P. Dynamics of the ascospore dispersal of Stagonosporopsis citrulli, a causal agent of gummy stem blight of cucurbits[J]. Plant Pathology, 2021, 00:1-12. https://doi.org/10.1111/ppa.13424.
[30] 张宁,毕研飞,郭静,等.不同抗性甜瓜接种蔓枯病菌后PAL、PPO与POD活性的变化[J].植物病理学报, 2016, 52(8):1169-1175.
[31] 鲁秀梅,张宁,夏美玲,等.不同抗性甜瓜接种蔓枯病菌后内源激素含量变化及其相关基因的表达分析[J].南京农业大学学报, 2018, 41(2):248-255.
[32] 任海英,李岗,方丽,等.不同甜瓜品种蔓枯病抗性与抗坏血酸过氧化物酶基因表达的关系[J].植物病理学报, 2012, 42(3):306-310.