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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 88-96.DOI: 10.16409/j.cnki.2095-039x.2021.08.004

• RESEARCH REPORTS • Previous Articles     Next Articles

Screening of Solid Fermentation Medium for Trichoderma asperellum Based on Polyurethane Sponge Carrier

YANG Xiaolong1, SHI Chaohui1, QIAO Yi1, YOU Zhonghao1, GU Gang2, LIN Zhihui3, ZHOU Ting2, LIANG Banjie3, CHEN Chengliang3, LIU Guokun1, XIAO Shun1   

  1. 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Institute of Tobacco Science Fujian provincial Tobacco Company, Fuzhou 350003, China;
    3. Sanming Company of Fujian Tobacco Company, Sanming 365000, China
  • Received:2021-07-05 Online:2022-02-08 Published:2022-03-01

Abstract: Using Trichoderma asperellum THGY-01 (CGMCC No:22422) as the test strain, we screened and optimized the type and concentration of spore-producing medium for solid fermentation on a polyurethane sponge carrier, and the elements that could promote sporulation were also identified. Using the spore production per unit mass of the polyurethane sponge carrier (spores/g) as an indicator, 11 types of carbon sources, 6 types of nitrogen sources and 14 types of inorganic salts (8 types containing large elements and 6 types containing trace elements) were screened by the single factor test method. The results showed that molasses was the best carbon source for sporulation; When fermentation with 5% (V/V) molasses for 6 days, the spore production reached 4.92×109 spores/g; When fermentation with 30.0% (V/V) for 8 days, the spore production even reached 18.86×109 spores/g, which was 11.45 times the highest spore production of other carbon sources. The best nitrogen source for sporulation was yeast powder. After 6 days of fermentation with 0.4% (W/V) yeast powder, the spore production reached 1.42×109 spores/g, which is 1.27 times the highest spore production of other nitrogen sources. We also found that adding a certain concentration of potassium ion-containing inorganic salt can increase the spore yield of Trichoderma solid fermentation on the polyurethane sponge carrier; with the addition of 0.20% (M/V) KH2PO4, the spore production reached 2.31×109 spores/g after 6 days of fermentation, which was 203 times higher than the control; Some trace elements such as Cu, Zn, and Mn at certain concentration also significantly promote the sporulation of tested strains. The above results provide a theoretical basis for the solid fermentation of Trichoderma asperellum on a polyurethane sponge carrier.

Key words: Trichoderma asperellum, polyurethane sponge, inert carrier, solid fermentation, medium

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