Optimization of Fermentation Medium Components and Conditions of Fusarium proliferatum Br-1 and Its Field Control Efficacy

doi:10.16409/j.cnki.2095-039x.2017.05.017

Abstract

Abstract: Biological control agent Br-1 was isolated from diseased broomrape and identified as Fusarium proliferatum. In order to increase the sporulation of strain Br-1, the medium components and fermentation conditions were optimized by single factor and orthogonal design. The results indicated that optimized medium components of strain Br-1 were glucose 1%, NH₄NO₃ 0.2%, KH₂PO₄ 0.3%, MgSO₄·7H₂O 0.1%. Optimum fermentation conditions of strain Br-1 were pH 5.0, medium capacity 50 mL/250 mL, temperature 24℃, rotating speed 190 r/min, fermentation time 6 d, with sporulation of Br-1 increased by 257.5 times in comparison with that under unoptimized conditions. Field trails showed that control efficacy of Br-1 on Orobanche cernua Loefling was up to 67.2%.

Key words: Fusarium proliferatum, fermentation condition, medium, biological control

CLC Number:

S476

WANG Yajiao, JI Lijing, LI Qiusheng, XIAO Ying, WANG Liansheng, KONG Lingxiao. Optimization of Fermentation Medium Components and Conditions of Fusarium proliferatum Br-1 and Its Field Control Efficacy[J]. journal1, 2017, 33(5): 692-698.

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