Synergistic Effect of Compound Seed Coating Containing Biocontrol Bacteria ZF516 and Chemical Agent on the Control of Cowpea Fusarium Root Rot

doi:10.16409/j.cnki.2095-039x.2025.02.010

Abstract

Abstract: In order to obtain biocontrol bacteria with remarkable antagonistic effects against cowpea root rot, in this study, the dural culture method was utilized to isolate a biocontrol strain, ZF516, which exhibited significant inhibitory effects on the complex pathogenic bacteria of cowpea root rot. Subsequently, the morphological characteristics of this strain were analyzed, and the 16S rRNA, gyrA, and rpoA gene sequences were compared and analyzed to clarify the taxonomic status of strain ZF516. The biosafety and root rot control efficacy of cowpea were evaluated through compatibility and compounding ratio screening of ZF516 with 23% imidacloprid·fludioxonil·difenoconazole and 29% thiamethoxam·fludioxonil·metalaxyl-M, respectively. The results indicated that the inhibition rates of strain ZF516 against Fusarium solani, F. oxysporum, and F. proliferatum were 71.83%, 66.31%, and 73.73%. The strain ZF516 was identificated as Bacillus velezensis. After formulating Bacillus velezensis ZF516 and 23% imidacloprid- thiamethoxam-benomyl at a volume ratio of 7:3, the emergence rate of cowpea seeds treated with this combined seed coating was significantly higher compared to untreated seeds and seeds treated with each agent individually. Additionally, the compound of Bacillus velezensis ZF516 with 23% imidacloprid·thiamethoxam·benomyl suspension seed coating agent showed a synergistic effect on the control of cowpea root rot, achieving a control efficiency of up to 67.56%, which surpassed the efficacy observed with the chemical seed coating agent alone. In this study, a strain of Bacillus velezensis ZF516 was isolated and characterized. ZF516 exhibited significant disease resistance against cowpea root rot, both as a standalone treatment and in combination with chemical agents. This study provides essential strain resources and integrated control strategies for the development of biological seed coating agents aimed at managing cowpea root rot.

Key words: seed coating agent, cowpea root rot, Bacillus, biocontrol efficiency

CLC Number:

S476

ZHAO Zixuan, SHI Bin, LANG Junying, JIANG Tao, YANG Jingjing, FAN Tengfei, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI Lei. Synergistic Effect of Compound Seed Coating Containing Biocontrol Bacteria ZF516 and Chemical Agent on the Control of Cowpea Fusarium Root Rot[J]. Chinese Journal of Biological Control, 2025, 41(1): 165-176.

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