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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 97-107.DOI: 10.16409/j.cnki.2095-039x.2021.08.005

• RESEARCH REPORTS • Previous Articles     Next Articles

Synergistic Effect of Seaweed Residue Combined with Microbial Inoculum on Cucumber Fusarium Wilt Control

HAO Dazhi1, WANG Yongkun1, CHEN Jie1, XIN Zhousheng2, Gao Yongdong3   

  1. 1. College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;
    2. Shandong Jie Jing Group Co., Ltd, Rizhao 276826, China;
    3. Shanghai Agricultural Technology Extension Service Center, Shanghai 201100, China
  • Received:2021-10-08 Online:2022-02-08 Published:2022-03-01

Abstract: The efficient utilization of seaweed residue is of great significance to environmental protection. This study aimed to understand the synergistic effects of seaweed residue, Trichoderma asperellum, and Bacillus amyloliquefaciens against cucumber Fusarium wilt by comparing seaweed residue and Trichoderma+Bacillus in single or in combination. The results showed that soil treatment with 1% seaweed residue+3.0×105cfu/g Trichoderma asperellum+2.0×108cfu/g Bacillus amyloliquefaciens (T1) could effectively improve the control effect against cucumber Fusarium wilt, with the efficacy of 93.39%. Compared with Trichoderma+Bacillus (T3), T1 increased the contol efficacy by 11.71%. After 9 days of inoculation with Fusarium oxysporum, compared with T3, T1 was more effective in the induction of POD, SOD, CAT, PPO and PAL enzyme activities in leaves and the accumulation of endogenous salicylic acid in roots and leaves, indicating that the addition of seaweed residue enhanced the control effect of Trichoderma and Bacillus on cucumber Fusarium wilt. The qPCR results showed that the addition of seaweed residue enhanced the ability of Trichoderma and Bacillus to induce defense response, and the expression level of SA pathway gene increased. Correlation analysis showed that the addition of seaweed residue was positively correlated with Trichoderma and Bacillus to induce the production of signal substances related to cucumber Fusarium wilt resistance and the expression of genes and enzyme activities. In conclusion, the major reason that the micro-ecological effect and systemic induced resistance effect against the cucumber Fusarium wilt was significantly generated by the synergistic action among seaweed residue, Trichoderma and Bacillus.

Key words: Trichoderma, Bacillus, seaweed residue, cucumber Fusarium wilt, induced systemic resistance

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