Development of Microbial Seed Coating Agents and Its Control Effect on Maize Stalk Rot

doi:10.16409/j.cnki.2095-039x.2024.02.046

Abstract

Abstract: In this study, Trichoderma strains with good control effects on maize stalk rot were screened by plate confrontation test, maize seed germination characteristics analysis and greenhouse pot experiments. The type, dosage and best combination of auxiliaries were determined by physicochemical properties, biocompatibility, and orthogonal tests. Control effect of the developed biological seed coating agents on maize stalk rot was verified. The results showed that 17 of 109 Trichoderma strains exhibited antagonistic activity against Fusarium graminearum with an average inhibitory rate of over 70%, among which 6 strains had the best control efficacy ranging from 80.33% to 85.64% on maize stalk rot at seedling stage. The active ingredients of two biological seed coating agents with T. asperellum strain Tr34 and T. afroharzianum strain Tr35 were developed, followed as 106 chlamydospores/mL, 0.2% xanthan gum, 2% sodium lignonate, 5% beinon film forming agent 1, and 1% 100 mg/mL streptomycin. When the ratios were 1:200 and 1:100, the biological seed coating agents Tr 34 and Tr 35 had the best control effect on maize stalk rot, with the efficacies of 81.14% and 83.18%, respectively. The biological seed coating agents Tr 34 and Tr 35 could effectively reduce the incidence of maize stalk rot, which lays a foundation for further field application.

Key words: biological seed coating agent, stalkrot, corn, Trichoderma, biological control

CLC Number:

S476

GUO Ning, MA Hongxia, ZHANG Haijian, SHI Jie, LIU Shusen, SUN Hua, ZHENG Xiaojuan. Development of Microbial Seed Coating Agents and Its Control Effect on Maize Stalk Rot[J]. Chinese Journal of Biological Control, 2024, 40(5): 1054-1065.

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