人参黑斑病生防菌的分离鉴定及防效

doi:10.16409/j.cnki.2095-039x.2022.02.033

摘要/Abstract

摘要： 本研究以人参链格孢为靶标菌，采用平板对峙法从人参根际土壤筛选出一株拮抗细菌YJ 3-7，结合细胞形态判断、16S rDNA和gyrA基因序列比对分析，鉴定菌株YJ 3-7为贝莱斯芽胞杆菌Bacillus velezensis。该菌株的发酵液、菌悬液和无菌发酵液对人参链格孢的生长均具有抑制作用，抑制率分别为98.76%、98.27%和70.62%。离体叶片和盆栽试验表明YJ 3-7发酵液可以较好地防治人参黑斑病，防效分别为63.24%和67.92%，盆栽防效略低于多菌灵(72.81%)。除此之外，菌株YJ 3-7还能有效抑制其他8种病原真菌的生长。研究表明贝莱斯芽胞杆菌YJ 3-7抑菌谱广，对人参黑斑病具有显著的防治效果，在植物病害防治中具有一定的应用潜力，可为人参黑斑病生物防治药剂的开发奠定基础。

关键词: 人参黑斑病, 贝莱斯芽胞杆菌, 生物防治

Abstract: An antagonistic bacterial strain YJ 3-7 was screened from the rhizosphere soil of ginseng by plate confrontation method using Alternaria panax as indicators.Combined with cell morphology,16S rDNA and gyrA gene sequence alignment analysis,the strain YJ 3-7 was identified as Bacillus velezensis.Fermentation broth,bacterial suspension and fermentation liquid without bacteria of strain YJ 3-7 all had inhibitory effects on the growth of A.panax,and the inhibition rates were 98.76%,98.27% and 70.62%,respectively.In vitro leaf experiment and pot experiment showed that YJ 3-7 fermentation broth could control ginseng black spot disease,with the control efficacies of 63.24% and 67.92%,respectively.The pot control effect of YJ 3-7 fermentation broth was slightly lower than that of carbendazim (72.81%).In addition,strain YJ 3-7 could effectively inhibit the growth of other 8 plant pathogens.The above results show that B.velezensis YJ 3-7 has broad-spectrum antagonistic effect,and obvious control effect on ginseng black spot disease,which laid a foundation for the development of bio-control agents against ginseng black spot disease.

Key words: ginseng black spot, Bacillus velezensis, biological control

中图分类号:

张宁, 张晶晶, 李雅淑, 李冉琪, 侯微, 曲正义, 李亚丽, 郑培和. 人参黑斑病生防菌的分离鉴定及防效[J]. 中国生物防治学报, 2022, 38(5): 1308-1315.

ZHANG Ning, ZHANG Jingjing, LI Yashu, LI Ranqi, HOU Wei, QU Zhengyi, LI Yali, ZHENG Peihe. Isolation,Identification and Control Effect of Biocontrol Bacteria against Ginseng Black Spot Disease[J]. Chinese Journal of Biological Control, 2022, 38(5): 1308-1315.

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