Abstract: Total 98 fungal strains isolated from the marine sponge Hymeniacidon perleve collected in Dalian, Liaoning, were screening for insecticidal and antimicrobial activities. A marine mycelial fungus strain Hmp-F73 was obtained based on brine shrimp assay. When the concentration of the methanolic extract from fermentation broth was 0.25 μg·mL^-1, the corrected mortality of brine shrimp was 100%. The methanolic extract was portioned with silica gel column chromatography, and 7 fractions were obtained. Fraction 3 at the concentration of 400 μg·mL^-1 could inhibit the feeding of Spodoptera exigu by the antifeedant rate of 85.25%. Fraction 6 at 1 mg·mL^-1 demonstrated significant inhibition activity against Rhizoctonia solani with 9.25 mm of inhibition zone. Insecticidal and antimicrobial metabolites were purified by bioassay guided fractionation, and further obtained monomeric compounds, and the chemical structure was established by the analysis of ¹H NMR data. The results suggested that these compounds all belonged to trichothecene mycotoxins. According to morphology and cultural characteristics and ITS1-5.8S-ITS2 sequence analysis, the strain Hmp-F73 was identified as Myrothecium verrucaria.