北苍术根区土壤中AM真菌多样性及其与土壤养分相关性分析

doi:10.16409/j.cnki.2095-039x.2021.06.019

中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (6): 1288-1297.DOI: 10.16409/j.cnki.2095-039x.2021.06.019

• 研究论文 • 上一篇

北苍术根区土壤中AM真菌多样性及其与土壤养分相关性分析

李铭杰^1,2, 周志杰³, 邢礼军², 尹鑫³, 武凤霞², 刘建斌², 邹国元², 张毅功¹, 张淑彬²

1. 河北农业大学资源与环境科学学院, 保定 071001;
2. 北京市农林科学院植物营养与资源研究所, 北京 100097;
3. 河北承德市农林科学院药用植物研究所, 承德 067000

收稿日期:2021-04-26 发布日期:2021-12-15
通讯作者: 邹国元,博士,研究员,E-mail:gyzou@163.com;张淑彬,硕士,副研究员,E-mail:zbinb@163.com。
作者简介:李铭杰,男,在读硕士研究生,E-mail:lmj3924@163.com。
基金资助:
自主立项（YZS201901）；河北省重点研发计划（20326419D）；北京市农林科学院科技创新能力建设专项（KJCX20200104，KJCX20190407）

Correlation Analysis between Diversity of AM Fungi and Nutrient in Root-zone Soil of Atractylodes chinensis

LI Mingjie^1,2, ZHOU Zhijie³, XING Lijun², YIN Xin³, WU Fengxia², LIU Jianbin², ZOU Guoyuan², ZHANG Yigong¹, ZHANG Shubin²

1. College of Resources and Environmental Sciences, Hebei Agricultural University, Baoding 071001, China;
2. Institute of Plant Nutrition and Resource, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China;
3. Institute of Medicinal plants, Chengde Academy of Agriculture and Forestry Sciences, Chengde 067000, China

Received:2021-04-26 Published:2021-12-15

摘要/Abstract

摘要： 本研究采用Illumina MiSeq高通量测序技术，对河北承德栽培和野生北苍术根区土壤AM真菌群落多样性的差异和群落组成进行了检测，同时解析了土壤养分因子对AM真菌群落多样性的影响。结果显示，北苍术根区土壤中检测的AM-OTU分属于1门1纲5目6科8属AM真菌。野生北苍术AM真菌群落由球囊霉属（77.20%）等组成；栽培北苍术由巨孢囊霉属（34.48%~47.20%）、球囊霉属（30.05%~53.7%）等组成；土壤有机质、pH、有效磷、有效钾与AM真菌多样性指数Shannon、Simpsoneven呈正相关，其中有机质对AM真菌群落多样性影响最大。硼与AM真菌多样性指数Shannon、Simpsoneven、Sobs均呈正相关，铁、铜、锰、锌与之呈负相关。本研究阐明了野生与栽培北苍术根区AM真菌多样性及优势菌群差异，揭示了土壤养分因子与其多样性间的关系，为AM真菌在北苍术仿野生栽培中的应用提供了理论依据。

关键词: 丛枝菌根真菌, 多样性, 高通量测序, 野生, 栽培

Abstract: In this study, the diversity and community compositon of AM fungi in root-zone soil of wild and cultivated Atractylodes chinensis (Chengde, Hebei province) was detected by the high throughput sequencing technology, and revealed the correlation between AM fungi diversity and nutrient factors. The results showed that the AM-OTU obtained from the root zone soil of wild and cultivated Atractylodes chinensis belonged to 8 genus, 6 families, 5 orders, 1 class, 1 phylum. The dominant AM fungi community of wild Atractylodes chinensis was composed of Glomus (77.20%), etc., The cultivated Atractylodes chinensis was composed of Gigaspora (34.48%-47.20%) and Glomus (30.05%-53.7%), etc.. Canonical correspondence analysis (CCA)showed that soil organic matter, pH value, available phosphorus, and available potassium were positively correlated with the Shannon index and Simpsoneven index of the AM fungal community, and organic matter showed the greatest impact on the diversity of the AM fungal community. The element B was positively correlated with the Shannon index, Simpsoneven index, and Sobs index of AM fungal community, and iron, copper, manganese, and zinc are negatively correlated with it.The results of this study interpreted the diversity of AM fungi and the difference of dominant fungi between wild and cultivated Atractylodes chinensis, revealed the relationship between soil nutrient factors and their diversity, which provided a basis for the application of AM fungi in wild-type cultivation of Atractylodes chinensis.

Key words: arbuscular mycorrhizal fungi, diversity, high throughput sequencing, cultivation, wild

中图分类号:

S182

李铭杰, 周志杰, 邢礼军, 尹鑫, 武凤霞, 刘建斌, 邹国元, 张毅功, 张淑彬. 北苍术根区土壤中AM真菌多样性及其与土壤养分相关性分析[J]. 中国生物防治学报, 2021, 37(6): 1288-1297.

LI Mingjie, ZHOU Zhijie, XING Lijun, YIN Xin, WU Fengxia, LIU Jianbin, ZOU Guoyuan, ZHANG Yigong, ZHANG Shubin. Correlation Analysis between Diversity of AM Fungi and Nutrient in Root-zone Soil of Atractylodes chinensis[J]. Chinese Journal of Biological Control, 2021, 37(6): 1288-1297.

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北苍术根区土壤中AM真菌多样性及其与土壤养分相关性分析

Correlation Analysis between Diversity of AM Fungi and Nutrient in Root-zone Soil of Atractylodes chinensis

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