Response of Rice Defense-related Genes to Blast Fungus

doi:10.16409/j.cnki.2095-039x.2017.04.010

Abstract

Abstract: The rice-Magnaporthe oryzae pathosystem has become a model in the study of plant-fungal interactions. The expression of several signal associated and PR genes were analyzed in six rice lines (harboring resistance genes Pik-s, Pita, Pit, Pi1, Pi9 respectively with the backcross parent LTH) under M. oryzae infection by RT-PCR. The results showed that the rice lines harboring blast resistance gene Pik-s, Pita, Pit were compatible interaction with M. oryzae #626, the rice lines harboring Pi1 and Pi9 were incompatible interaction. The expression of MAPK12 and MAPK6 were up-regulated under susceptible interaction. BIMK2 showed higher expression under Pi9 mediated incompatible interaction. In conclusion, the PR genes expression pattern was more diversity to M. oryzae infection in harboring different resistance genes in rice monogenic lines. The up-regulation response of PR genes appeared in the early infection period in incompatible interactions, and at the late period in compatible interactions, indicating that the temporal expression pattern of these PR genes play an important role in plant pathogen interactions.

Key words: rice blast disease, defense-related gene, rice near isogenic line, interaction

CLC Number:

S435.11

LIU Chao, WANG Hui, LIU Lin, YANG Jing, LI Chengyun. Response of Rice Defense-related Genes to Blast Fungus[J]. journal1, 2017, 33(4): 504-511.

References

[1] van Loon L C, Rep M, Pieterse C M. Significance of inducible defense-related proteins in infected plants[J]. Annual Review of Phytopathology, 2006, 44:135-162.
[2] Yuan Y, Zhong S, Li Q, et al. Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility[J]. Plant Biotechnology Journal, 2007, 5(2):313-324.
[3] Kaku H, Nishizawa Y, Ishii-Minami N, et al. Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor[J]. Proceedings of the National Academy of Sciences, 2006, 103(29):11086-11091.
[4] Liu B, Li J F, Ao Y, et al. Lysin motif-containing proteins LYP4 and LYP6 play dual roles in peptidoglycan and chitin perception in rice innate immunity[J]. The Plant Cell, 2012, 24(8):3406-3419.
[5] Sinha A K, Jaggi M, Raghuram B, et al. Mitogen-activated protein kinase signaling in plants under abiotic stress[J]. Plant Signaling and Behavior, 2011, 6(2):196-203.
[6] Adachi H, Ishihama N, Nakano T, et al. Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea[J].Plant Signaling and Behavior, 2016, 11(6):e1183085.
[7] Birkenbihl R P, Kracher B, Somssich I E. Induced genome-wide binding of three Arabidopsis WRKY transcription factors during early MAMP-triggered immunity[J]. The Plant Cell, 2017, 29(1):20-38.
[8] Shimono M, Koga H, Akagi A Y A, et al. Rice WRKY45 plays important roles in fungal and bacterial disease resistance[J]. Molecular Plant Pathology, 2012, 13(1):83-94.
[9] Pandey S P, Somssich I E. The role of WRKY transcription factors in plant immunity[J]. Plant Physiology, 2009, 150(4):1648-1655.
[10] Wang H, Hao J, Chen X, et al. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants[J]. Plant Molecular Biology, 2007, 65(6):799-815.
[11] Breen S, Williams S J, Winterberg B, et al. Wheat PR-1 proteins are targeted by necrotrophic pathogen effector proteins[J]. The Plant Journal, 2016, 88(1):13-25.
[12] Bufe A, Spangfort M D, Kahlert H, et al. The major birch pollen allergen, Bet v 1, shows ribonuclease activity[J]. Planta, 1996, 199(3):413-415.
[13] Liu C, Cheng F, Sun Y, et al. Structure-function relationship of a novel PR-5 protein with antimicrobial activity from soy Hulls[J]. Journal of Agricultural and Food Chemistry, 2016, 64(4):948-959.
[14] Mahdavi F, Sariah M, Maziah M. Expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to Fusarium wilt[J]. Applied Biochemistry and Biotechnology, 2012, 166(4):1008-1019.
[15] Misra R C, Sandeep M K, Kumar S, et al. A thaumatin-like protein of Ocimum basilicum confers tolerance to fungal pathogen and abiotic stress in transgenic Arabidopsis[J]. Scientific Reports, 2016, 6:25340
[16] 杨祁云, 朱小源, 雷财林, 等. 华南籼稻稻瘟病菌致病型单基因鉴别寄主筛选[J]. 植物保护学报, 2004, 31(2):113-120.
[17] 李进斌, 李成云, 张庆, 等. 二十二个抗稻瘟病基因在云南的利用价值评价[J]. 植物保护学报, 2005, 32(2):113-119.
[18] 杨健源, 陈深, 曾列先, 等. 稻瘟病主效抗性基因对广东省籼稻稻瘟病菌的抗性评价[J]. 中国水稻科学, 2008, 22(2):190-196.
[19] Shimizu T, Nakano T, Takamizawa D, et al. Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice[J]. The Plant Journal, 2010, 64(2):204-214.
[20] Kim S H, Oikawa T, Kyozuka J, et al. The bHLH Rac immunity1(RAI1) is activated by OsRac1 via OsMAPK3 and OsMAPK6 in rice immunity[J]. Plant and Cell Physiology, 2012, 53(4):740-754.
[21] Lieberherr D, Thao N P, Nakashima A, et al. A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice[J]. Plant Physiology, 2005, 138(3):1644-1652.
[22] Yoo S D, Cho Y H, Tena G, et al. Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling[J]. Nature, 2008, 451(7180):789-795.
[23] Kawano Y, Akamatsu A, Hayashi K, et al. Activation of a Rac GTPase by the NLR family disease resistance protein Pit plays a critical role in rice innate immunity[J]. Cell Host and Microbe, 2010, 7(5):362-375.