Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal Of Biological Control ›› 2019, Vol. 35 ›› Issue (6): 915-921.DOI: 10.16409/j.cnki.2095-039x.2019.06.019

• RESEARCH REPORTS • Previous Articles     Next Articles

Colonization Dynamics Marine Bacterium Bacillus amyloliquefaciens BA-3 and Its Impact on the Microbial Community of Cymbidium Rhizosphere

YAO Jinai, HUANG Peng, HOU Xiangyu, YU Deyi   

  1. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Science, Fuzhou 350013, China
  • Received:2019-07-31 Online:2019-12-08 Published:2019-12-18

Abstract: Marine bacterium strain BA-3 was applied to cymbidium plants to investigate the colonization features and the impact on the microbial community of Cymbidium rhizosphere. Mutant strain BA-3-K with stable marker of antibiotic and inhibitory effect against Fusarium oxysporium of cymbidium were screened by using the rifampin and kanamycin-resistant mutants and dual culture method. Plate confrontation test proves that the inhibition rates of strain BA-3-K and original strain BA-3 against Fusarium oxysporium were 86.91% and 87.69%. It was verified that the strain BA-3-K could colonize for 60 days or more in cymbidium by root irrigating and stem daubing. After root irrigating, the colonization quantity of strain BA-3-K trended to increase firstly and then decrease. The highest quantity in roots and stems were 2.54×105 cfu/g and 1.47×105 cfu/g at 21 d, in soil were 6.50×105 cfu/g at 15 d after inoculation, respectively. Bacteria quantities in different parts were soil > root > stem and strain BA-3-K wasn't detected in leaf. After stem daubing, the highest colonization quantity in stem was 2.33×105 cfu/g at 17 d after inoculation and then decreased. Bacteria quantities in different parts were stem>leaf. and strain BA-3-K wasn't detected in root and soil. The strain BA-3-K could colonize in stem of cymbidium by scanning electron microscope. Pot tests demonstrated that the quantities of bacteria, fungi and actinomycetes in rhizosphere soil was significantly higher than that in control by root irrigating with strain BA-3-K. The results indicated that marine bacterium strain BA-3 has strong colonization ability and good application value.

Key words: marine bacteria, Bacillus amyloliquefaciens, stem rot on cymbidium, colonization

CLC Number: