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journal1 ›› 2018, Vol. 34 ›› Issue (3): 431-439.DOI: 10.16409/j.cnki.2095-039x.2018.03.005

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Antagonistic Effect of the Mixed Bacteria Culture on Verticillium dahlia

LI Guo1, ZHAO Hui1, LIU Yuanyuan1, ZHANG Mengtian1, PANG Xuebing1, GUO Jianqiang1, LI Xiangyu2, ZHU Jianbo1, WANG Aiying1   

  1. 1. Key Laboratory of Agricultural Biotechnology/College of Life Science, Shihezi University, Shihezi 832000, China;
    2. Agricultural Division seven Mission Forestry Station, Aksu 842000, China
  • Received:2017-10-16 Online:2018-06-08 Published:2018-06-14

Abstract: Bacillus tequilensis C-9 and Sphingobacterium A1 isolated in this research had good biocontrol effect on Verticillium dahliae of cotton. The antimicrobial rates of the isolates C-9 and A1 were 77.8% and 83.3%, respectively. Related gene fragments Surfactin, Fengycin and Mycosubtilins were detected in both strains by PCR amplification. Single factor experiment showed that the best ratio of mixed bacteria A1and C-9 was 1:9. At this ratio, the biocontrol ability of V. dahliae was 131% and 36.7%, respectively, higher than that of single strain. Microscopic observation demonstrated that the mixed culture can cause V. dahlia mycelium swelling and deformed. Pot experiments showed that the control effect of the mixed culture was up to 66.7%. The cotton plants treated with the mixed culture showed a rapid response in CAT activity, and the peak value increased by 135.3% compared with CK in the same period. The activities of POD and SOD were increased by 22.5% and 39.8%, respectively, while the content of MDA was always lower than CK. Comprehensive analysis showed that the fermentation of mixed bacteria could improve the inhibition effect on V. dahliae and enhance the resistance by directly inhibiting the pathogen growth and inducing plant defense reaction.

Key words: mixed culture, Verticillium dahliae, induced resistance

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