Antagonistic Effect of the Mixed Bacteria Culture on Verticillium dahlia

doi:10.16409/j.cnki.2095-039x.2018.03.005

Abstract

Abstract: Bacillus tequilensis C-9 and Sphingobacterium A1 isolated in this research had good biocontrol effect on Verticillium dahliae of cotton. The antimicrobial rates of the isolates C-9 and A1 were 77.8% and 83.3%, respectively. Related gene fragments Surfactin, Fengycin and Mycosubtilins were detected in both strains by PCR amplification. Single factor experiment showed that the best ratio of mixed bacteria A1and C-9 was 1:9. At this ratio, the biocontrol ability of V. dahliae was 131% and 36.7%, respectively, higher than that of single strain. Microscopic observation demonstrated that the mixed culture can cause V. dahlia mycelium swelling and deformed. Pot experiments showed that the control effect of the mixed culture was up to 66.7%. The cotton plants treated with the mixed culture showed a rapid response in CAT activity, and the peak value increased by 135.3% compared with CK in the same period. The activities of POD and SOD were increased by 22.5% and 39.8%, respectively, while the content of MDA was always lower than CK. Comprehensive analysis showed that the fermentation of mixed bacteria could improve the inhibition effect on V. dahliae and enhance the resistance by directly inhibiting the pathogen growth and inducing plant defense reaction.

Key words: mixed culture, Verticillium dahliae, induced resistance

CLC Number:

S476

LI Guo, ZHAO Hui, LIU Yuanyuan, ZHANG Mengtian, PANG Xuebing, GUO Jianqiang, LI Xiangyu, ZHU Jianbo, WANG Aiying. Antagonistic Effect of the Mixed Bacteria Culture on Verticillium dahlia[J]. journal1, 2018, 34(3): 431-439.

References

[1] Woolliams G E. Host range and symptomatology of Verticillium dahliae in economic,wee[J]. Canadian Journal of Plant Science, 1966, 46(6):661-669.
[2] Krikun J, Bernier C C. Infection of several crop species by two isolates of Verticillium dahliae[J]. Canadian Journal of Plant Pathology, 1987, 9(3):241-245.
[3] Pegg G F, Brady B L, Pegg G F, et al. Control[J]. Purchasing and Supply Management, 2002, 22(11):43-53.
[4] Wang F X, Ma Y P, Yang C L, et al. Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae[J]. Proteomics, 2011, 11(22):4296-4309.
[5] Cabanás C G, Schilirò E, Valverdecorredor A, et al. The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots[J]. Frontiers in Microbiology, 2014, 5:427.
[6] Berg G. Plant-microbe interactions promoting plant growth and health:perspectives for controlled use of microorganisms in agriculture[J]. Applied Microbiology and Biotechnology, 2009, 84(1):11-18.
[7] Cabanás C G, Schilirò E, Valverdecorredor A, et al. The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots[J]. Frontiers in Microbiology, 2014, 5:427.
[8] 葛红莲, 郭坚华, 祁红英, 等. 复合菌剂AR99防治辣椒青枯病[J]. 植物病理学报, 2004, 34(2):162-165.
[9] 申爱荣, 姬广海, 魏兰芳, 等. 复合菌剂防治马铃薯青枯病研究[J]. 云南农业大学学报, 2006, 21(4):449-453.
[10] 谭兆赞, 林捷, 刘可星, 等. 复合微生物菌剂对番茄青枯病和土壤微生物多样性的影响[J]. 华南农业大学学报, 2007, 28(1):45-49.
[11] 许艳丽, 张红骥, 张匀华, 等. 复配生防菌株防治大豆根腐病的研究[J]. 大豆科学, 2008, 27(2):270-274.
[12] Linderman R G. Vesicular-Arbuscular Mycorrhizae and Soil Microbial Interactions[M]. Asa Special Publication, 1992, 45-70.
[13] Barea J M, Azconaguilar C. Interactions between Mycorrhizal Fungi and Rhizosphere Microorganisms within the Context of Sustainable Soil-plant Systems[M]//Gange A C, Brown V K (eds). Multitrophic Interactions in Terrestrial Systems, Oxford:Blackwell Science, 1997, 65-77.
[14] Saldajeno M G B, Chandanie W A, Kubota M, et al. Effects of Interactions of Arbuscular Mycorrhizal Fungi and Beneficial Saprophytic Mycoflora on Plant Growth and Disease Protection[M]. Mycorrhizae:Sustainable Agriculture and Forestry, 2008, 211-226.
[15] Martínez-Medina A, Pascual J A, Lloret E, et al. Interactions between arbuscular mycorrhizal fungi and Trichoderma harzianum and their effects on Fusarium wilt in melon plants grown in seedling nurseries[J]. Journal of the Science of Food and Agriculture, 2009, 89(11):1843-1850.
[16] Davelos A L, Kinkel L L, Samac D A. Spatial Variation in Frequency and Intensity of Antibiotic Interactions among Streptomycetes from Prairie Soil[J]. Applied and Environmental Microbiology, 2004, 70(2):1051-1058.
[17] Mde B, Bom P, Kindt F, et al. Control of Fusarium wilt of radish by combining Pseudomonas putida strains that have different disease-suppressive mechanisms[J]. Phytopathology, 2003, 93(5):626-632.
[18] Guetsky R, Shtienberg D, Elad Y, et al. Combining biocontrol agents to reduce the variability of biological control[J]. Phytopathology, 2001, 91(7):621.
[19] Kusari S, Hertweck C, Spiteller M. Chemical ecology of endophytic fungi:origins of secondary metabolites[J]. Chemistry and Biology, 2012, 19(7):792-798.
[20] 曹明慧, 冉炜, 杨兴明, 等. 烟草黑胫病拮抗茵的筛选及其生物效应[J]. 土壤学报, 2011(1):151-159.
[21] Baffoni L, Gaggia F, Dalanaj N, et al. Microbial inoculants for the biocontrol of Fusarium spp in durum wheat[J]. Bmc Microbiology, 2015, 15(1):242.
[22] Tapi A, Chollet-Imbert M, Scherens B, et al. New approach for the detection of non-ribosomal peptide, synthetase genes in Bacillus strains by polymerase,chain reaction[J]. Applied Microbiology and Biotechnology, 2010, 85(5):1521-1531.
[23] Alvarez F, Castro M, Príncipe A, et al. The plant-associated Bacillus amyloliquefaciens strains MEP218 and ARP23 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease[J]. Journal of Applied Microbiology, 2012, 112(1):159-174.
[24] 陈红, 王玲霞. 混合菌培养提高水稻纹枯病生防效果的研究[J]. 中国农学通报, 2001, 17(5):1-5.
[25] 柴红玉, 王潇, 卢富山, 等. 乳酸链球菌和干酪乳杆菌混合培养抑菌性的研究[J]. 江西农业学报, 2015(2):85-87.
[26] Tao Y, Bie X M, Lv F X, et al. Antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against Rhizopus stolonifer[J]. Journal of Microbiology, 2011, 49(1):146-150.
[27] Han Q, Wu F, Wang X, et al. The bacterial lipopeptide iturins induce Verticillium dahliae cell death by affecting fungal signalling pathways and mediate plant defence responses involved in pathogen-associated molecular pattern-triggered immunity[J]. Environmental Microbiology, 2015, 17(4):1166-88.
[28] Kim S Y, Ko Y J, Jung K W, et al. Hrk1 plays both hog1-dependent and -independent roles in controlling stress response and antifungal drug resistance in Cryptococcus neoformans[J]. Plos ONE, 2011, 6(4), e18769.
[29] Pantelides I S, Tjamos S E, Paplomatas E J. Ethylene perception via ETR1 is required in Arabidopsis infection by Verticillium dahliae[J]. Molecular Plant Pathology, 2010, 11(2):191.
[30] Johansson A, Staal J, Dixelius C. Early responses in the Arabidopsis-Verticillium longisporum pathosystem are dependent on NDR1, JA-and ET-associated signals via cytosolic NPR1 and RFO1[J]. Molecular Plant-microbe Interactions:MPMI, 2006, 19(9):958.
[31] 周京龙, 冯自力, 冯鸿杰, 等. 棉花内生蜡状芽孢杆菌YUPP-10对棉花黄萎病的防治作用及机制[J]. 中国农业科学, 2017, 50(14):2717-2727.
[32] 张文婷, 张秋丽, 冀媛媛, 等. 特殊生境中植物枯、黄萎病拮抗放线菌的筛选及鉴定[J]. 西北农林科技大学学报(自然科学版), 2011, 39(9):187-192.