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journal1 ›› 2019, Vol. 35 ›› Issue (1): 99-111.DOI: 10.16409/j.cnki.2095-039x.2019.01.018

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The Standards and Methods for Efficient Evaluation of Tolerance of Antagonistic Trichodema Strains

WANG Qianqiang1, DOU Kai1, CHEN Jie1, JIANG Xiliang2, YANG Hetong3, WANG Wei4   

  1. 1. State Laboratory of Microbiology Metabolism/Key Laboratory of Urban Agriculture(South), Ministry of Agriculture/College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;
    2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Ecology Institute of Shandong Academy of Sciences, Jinan 250014, China;
    4. State Key Laboratory of Bioreactor Engineering/Institute of Bioegineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2017-11-18 Online:2019-02-08 Published:2019-01-31

Abstract: Efficient screening of Trichoderma strains with biocontrol potential and tolerance to various environment stress factors including extreme temperature, salt and drought etc was extensively required. Hence in this work, we examined a total of 20 Trichoderma isolates from five species in the tolerance performance to different stress conditions, including high and/or low temperature, salt and drought, and then established the threshold and standardized evaluation method based on colony growth and selected biochemical factors activity/contents of Trichoderma strains at different levels of each stress factor. Eventually the correlation of colony growth changes of Trichoderma spp. with the selected enzymes activity or MDA contents was analyzed under different stress conditions, and the correlation between spore germination rate and colony diameter under the corresponding stress was also analyzed. These results showed that the thresholds for Trichoderma growth response to stress factors were 1 mol/L of NaCl (salt stress), 36℃ (high temperature), 14℃ (low temperature) and 400 g/L PEG-6000 (drought stress). The diameter of colony was positively correlated with the spore germination rate under the corresponding stress. Most of the selected biochemical factors activity indexes under different stresses factors were significantly correlated with the dry weight of mycelium. In conclusion, the establishment of threshold of Trichoderma strains resistant to different stresses factors provided the essential basis for scientific evaluation of Trichoderma, which is important for evaluation and utilization of tolerant Trichoderma resources as biocontrol agents.

Key words: Trichoderma, tolerance, evaluation threshold

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