The Standards and Methods for Efficient Evaluation of Tolerance of Antagonistic Trichodema Strains

doi:10.16409/j.cnki.2095-039x.2019.01.018

Abstract

Abstract: Efficient screening of Trichoderma strains with biocontrol potential and tolerance to various environment stress factors including extreme temperature, salt and drought etc was extensively required. Hence in this work, we examined a total of 20 Trichoderma isolates from five species in the tolerance performance to different stress conditions, including high and/or low temperature, salt and drought, and then established the threshold and standardized evaluation method based on colony growth and selected biochemical factors activity/contents of Trichoderma strains at different levels of each stress factor. Eventually the correlation of colony growth changes of Trichoderma spp. with the selected enzymes activity or MDA contents was analyzed under different stress conditions, and the correlation between spore germination rate and colony diameter under the corresponding stress was also analyzed. These results showed that the thresholds for Trichoderma growth response to stress factors were 1 mol/L of NaCl (salt stress), 36℃ (high temperature), 14℃ (low temperature) and 400 g/L PEG-6000 (drought stress). The diameter of colony was positively correlated with the spore germination rate under the corresponding stress. Most of the selected biochemical factors activity indexes under different stresses factors were significantly correlated with the dry weight of mycelium. In conclusion, the establishment of threshold of Trichoderma strains resistant to different stresses factors provided the essential basis for scientific evaluation of Trichoderma, which is important for evaluation and utilization of tolerant Trichoderma resources as biocontrol agents.

Key words: Trichoderma, tolerance, evaluation threshold

CLC Number:

S476

WANG Qianqiang, DOU Kai, CHEN Jie, JIANG Xiliang, YANG Hetong, WANG Wei. The Standards and Methods for Efficient Evaluation of Tolerance of Antagonistic Trichodema Strains[J]. journal1, 2019, 35(1): 99-111.

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