Virulence of Metarhizium anisopliae against 3rd Instar Nymphs of Locusta migratoria manilensis under Different Temperatures

doi:10.16409/j.cnki.2095-039x.2019.04.021

Abstract

Abstract: The 3^rd instar nymphs of Locusta migratoria manilensis were treated by the commercial biopesticides Metarhizium anisopliae (PD20080671) through body surface inoculation under six different temperatures (15, 20, 25, 30, 35 and 40℃). The results showed that the cumulative mortality of locust nymphs increased within 19 d after inoculation. The highest (82.5%) was at 35℃ and the lowest (55.0%) was at 40℃. Most of the dead locusts were 3^rd instars and a few were adults as the nyphms molted during the test period. Spore germination from of the dead locusts appeared sooner (4.26 d) at 40℃ and took longer (19.36 days) at 15℃. The results indicate that temperature below 15℃ and greater than 35℃ are not optimal for fungal pathogenesis. These results demonstrate that the best time to apply biopesticides is early morning when the temperature is relatively low.

Key words: biopesticides product, Metarhizium anisopliae, Locust migratoria manilensis, nymph

CLC Number:

S476.1

LIU Yinmin, CHENG Yumeng, LI Hongmei, NONG Xiangqun, Belinda LUKE. Virulence of Metarhizium anisopliae against 3^rd Instar Nymphs of Locusta migratoria manilensis under Different Temperatures[J]. Chinese Journal Of Biological Control, 2019, 35(4): 642-647.

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Virulence of Metarhizium anisopliae against 3^rd Instar Nymphs of Locusta migratoria manilensis under Different Temperatures

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