Control Effect of Mixed Inoculation of Different Biocontrol Strains on Botrytis cinerea

doi:10.16409/j.cnki.2095-039x.2020.02.01

Abstract

Abstract: Inhibition effect of Bacillus subtilis GLB191 and B. pumilus GLB197 single strain and mixed strains against B. cinerea was investigated by inhibition zone test, inhibition test of conidia germination and in vitro inoculation on fruit. The results showed that the inhibition zone size of each treatment was more than 30 mm, and the inhibition rate of spore germination was more than 94%. The mixture of the two strains with 500 times dilution inhibited the spore germination higher than single strain. In vitro inoculation on fruit test showed that the lesion inhibition rate of the mixed strains treatment was up to 70.89%,higher than that of the single strain GLB191 and GLB197, which was 55.48% and 52.91%, respectively. Field trials indicated that control efficiency of the mixed strains was no significant difference with chemical agents, with the efficacy of 70.02%. Therefore, mixed inoculation of the two strains can be applied in grape grey mold control.

Key words: Botrytis cinerea, Bacillus, mixed inoculation, inhibitory effect, field efficacy

CLC Number:

S476

WANG Hongli, SHAN Wenhui, HU Haiyao, LI Yan, WANG Qi, WANG Kun, BIAN Fenge. Control Effect of Mixed Inoculation of Different Biocontrol Strains on Botrytis cinerea[J]. Chinese Journal Of Biological Control, 2020, 36(2): 265-271.

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