Screening for Bacillus thuringiensis Strains with High Toxicity against Spodoptera frugiperda

doi:10.16409/j.cnki.2095-039x.2019.05.028

Abstract

Abstract: To screen Bt strains with high virulence against Spodoptera frugiperda is crucial for the development of green and efficient IPM of S. frugiperda. In this study, nine Bt strains with high toxicity to Noctuidae, isolated by our company, were tested for their virulence against S. frugiperda in laboratory, and analyzed the insecticidal genes. Three of the highest virulent strains were further tested in field trial for their control efficacies against S. frugiperda. The results indicated that nine Bt strains showed prominent insecticidal activity against neonate of S. frugiperda at 7 days post treatment. The LC₅₀ ranged from 0.07 to 3.38 μg/g. The three highest virulent strains were KN50, KN11 and KNR8, with LC₅₀ of 0.07, 0.23 and 0.43 μg/g, respectively, showing very remarkable efficacy. Insecticidal gene analysis results demonstrated that six highly virulent Bt strains contained the same four insecticidal genes-vip3Aa, cry2Ab, cry1Ac and cry1Ia. Field trial results showed that the control efficacies of KN50, KN11 and KNR8 with 32000 IU/mg were significantly different, but were consistent with laboratory results. KN50 showed the highest insecticidal efficacy at 7 days post treatment, which was 86.09% and 95.22% for 200 g/667 m² and 400 g/667 m², respectively, against the first to third instar larvae, and 72.61% and 86.59% against larval populations of mixed instars. Therefore, the KN50, KN11 and KNR8 strains represent candidates for new product development to replace chemical pesticides and achieve green control S. frugiperdar.

Key words: Spodoptera frugiperda, Bacillus thuringiensis, bioassay, insecticidal gene analysis, field control efficacy

CLC Number:

S476.1

LIU Huamei, HU Xiao, WANG Yinglong, YANG Puyun, SHU Changlong, ZHU Xiaoming, ZHANG Jie, SUN Gangzhong, ZHANG Xiaoming, LI Qing. Screening for Bacillus thuringiensis Strains with High Toxicity against Spodoptera frugiperda[J]. Chinese Journal Of Biological Control, 2019, 35(5): 721-728.

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