Control Effects of Tank Mixtures of Chlorantraniliprole, Avermectin and Bacillus thuringiensis against Plutella xylostella in Different Areas

doi:10.16409/j.cnki.2095-039x.2017.06.003

Abstract

Abstract: We find out the resistance status of chlorantraniliprole, avermectin, and Bacillus thuringiensis, and field control effect of tank mixtures of 3 insecticides against different Plutella xylostella field populations in Tonghai Yunnan Province, Wuhan Hubei Province, and Haikou Hainan Province, to provide a reference for the strategy of resistance management to P. xylostella, and promote chemical pesticide reduction and control. The resistance and field efficacy of the 3 insecticides were tested by leaf dipping bioassay and field trial. The results indicated that 3 field population of P. xylostella have produced a high level of resistance to avermectin, with the resistance ratios of 276~1062 fold. The resistance ratios to chlorantraniliprol were 60~671 fold, which tested moderate to high level of resistance. Moderate and low level of resistance to B. thuringiensis was found with the resistance ratios of 5.1~15.2 fold. The field control effect and resistance level displayed a consistent trend. The effect of B. thuringiensis reached more than 70% after 7 days, while the chlorantraniliprole and avermectin were less than 60%. The field control effects of tank mixtures of chlorantraniliprole, avermectin and B. thuringiensis were 88.70% and 81.50% in the above areas respectively. The difference between the two tank mixtures and their single agents was significant. Therefore, it was recommended to mix or to rotate using B. thuringiensis in order to improve the control effect of P. xylostella.

Key words: Plutella xylostella, resistance, Bacillus thuringiensis, tank mixture, field effect

CLC Number:

S476.1

HU Xiao, WANG Xiaohui, SUN Gangzhong, ZHOU Li, PAN Yu, LIU Shoude, LI Qing, JIN Haiyan, YIN Fei, LI Zhenyu, CHEN Aidong, XIE Shenghua, LIU Huamei. Control Effects of Tank Mixtures of Chlorantraniliprole, Avermectin and Bacillus thuringiensis against Plutella xylostella in Different Areas[J]. journal1, 2017, 33(6): 732-738.

References

[1] Furlong M J, Wright D J, Dosdall L M. Diamondback moth ecology and management:problems, progress, and prospects[J]. Annual Review of Entomology, 2013, 58:517-541.
[2] 吴青君, 张文吉, 朱国仁. 小菜蛾的发生为害特点及抗药性现状[J]. 中国蔬菜, 2001(5):49-51.
[3] 赵建周, 吴世昌, 顾言真, 等. 小菜蛾抗药性治理对策研究[J]. 中国农业科学, 1996, 29(1):8-14.
[4] 胡珍娣, 冯夏, 李振宇, 等. 不同小菜蛾田间种群对氯虫苯甲酰胺药剂的敏感性[J]. 农药研究与应用, 2010, 14(3):25-27.
[5] 李向永, 尹艳琼, 赵雪晴, 等. 通海菜区小菜蛾的主要生物学特征[J]. 动物学研究, 2011, 32(5):48-51.
[6] Zalucki M P, Shabbir A, Silva R, et al. Estimating the economic cost of one of the world's major insect pests, Plutella xylostella (Lepidoptera:Plutellidae):just how long is a piece of string?[J]. Journal of Economic Entomology, 2012, 105(4):1115-1129.
[7] 梁沛, 高希武, 郑炳宗, 等. 小菜蛾对阿维菌素的抗性机制及交互抗性研究[J]. 农药学学报, 2001, 3(1):41-45.
[8] 冯夏, 李振宇, 吴青君, 等. 小菜蛾抗性治理及可持续防控技术研究与示范——公益性行业(农业)科研专项"小菜蛾可持续防控技术研究与示范"[J]. 应用昆虫学报, 2011, 48(2):247-253.
[9] 肖春雷, 罗丰, 刘勇, 等. 氯虫苯甲酰胺对小菜蛾的室内毒力测定及防治效果[J]. 中国蔬菜, 2012(16):86-89.
[10] 杨桂秋, 童怡春, 杨辉斌, 等. 新型杀虫剂氯虫苯甲酰胺研究概述[J]. 世界农药, 2012, 34(1):31-34.
[11] Horowitz A R, Ishaaya I. Biorational insecticides-mechanisms, selectivity and importance in pest managemen//Horowitz A R, Ishaaya I, eds. Insect Pest Management[M]. Berlin, Heidelberg:Springer, 2004, 1-28.
[12] 尹艳琼, 沐卫东, 李向永, 等. 云南通海小菜蛾种群抗药性监测及田间药效评价[J]. 植物保护, 2015, 41(3):205-209.
[13] 尹飞, 冯夏, 李振宇, 等. 不同地区小菜蛾对氯虫苯甲酰胺的抗药性差异[J]. 植物保护, 2015, 41(5):160-163.
[14] 胡珍娣. 小菜蛾对氯虫苯甲酰胺的抗药性及其解毒机理研究[D]. 北京:中国农业大学, 2016, 15-17.
[15] 罗雁婕, 高希武, 吴文伟, 等. 药剂对小菜蛾抗性及敏感品系乙酰胆碱酯酶抑制作用比较[J]. 农药学学报, 2008, 10(2):211-216.
[16] Sanchis V. From microbial sprays to insect-resistant transgenic plants:history of the biospesticide Bacillus thuringiensis. A review[J]. Agronomy for Sustainable Development, 2011, 31(1):217-231.
[17] Raymond B, Johnston P R, Nielsen L C, et al. Bacillus thuringiensis:an impotent pathogen?[J]. Trends in Microbiology, 2010, 18:189-194.
[18] Bravoa A, Likitvivatanavongb S, Gillb S S, et al. Bacillus thuringiensis:A story of a successful bioinsecticide[J]. Insect Biochemistry and Molecular Biology, 2011, 41(7):423-431.
[19] 周荣华, 刘翠君, 杨天武, 等. Bt杀虫剂与化学农药混用的研究概况[J]. 湖北农业科学, 2004(5):54-57.
[20] 徐健, 蔡扬生, 朱锦磊, 等. 苏云金杆菌与化学杀虫剂的联合作用测定[J]. 江苏农业科学, 2001(2):45-46.
[21] 吴华, 黄鸿, 欧剑峰, 等. 小菜蛾颗体病毒与苏云金杆菌混合悬浮剂防治小菜蛾的田间药效试验[J]. 昆虫天敌, 2004, 26(3):105-107.
[22] 石志琦, 严清平, 王春梅, 等. 蛇床子素和Bt复配对小菜蛾防治作用研究[J]. 上海农药学报, 2006, 22(2):58-60.
[23] 张聪冲, 邱浩, 覃春华, 等. Bt工程菌WG-001与化学杀虫剂复配最佳配方筛选及田间防效研究[J]. 应用昆虫学报, 2011, 48(2):313-318
[24] 邱德文. 生物农药的发展现状与趋势分析[J]. 中国生物防治学报, 2015, 31(5):679-684.
[25] 梁永亮, 康晓慧, 周强, 等. 2种生物农药对油菜菜青虫和小菜蛾的防治效果[J]. 中国植保导刊, 2017, 37(7):53-55.
[26] 郭白洁, 彭建新, 蔡旭. 乙基多杀菌素和苏云金杆菌复配防治小菜蛾田间药效评价[J]. 福建农业科技, 2016(7):22-24.
[27] 韩新才, 黄志农, 蔡福民, 等. 几种Bt复配杀虫剂对甘蓝小菜蛾的防治效果[J]. 湖北农业科学, 2004(6):44-46.
[28] Shelton A M, Robertson J L, Tang J D, et al. Resistance of diamondback moth (Lepidoptera:Plutellidae) to Bacillus thuringiensis subspecies in the field[J]. Journal of Economic Entomology, 1993, 86(3):697-705.
[29] 唐启义, 冯明光. DPS数据处理系统[M]. 北京:科学出版社, 2006.
[30] 姜兴印, 王开运, 仪美芹. 不同地区小菜蛾对杀虫剂的抗性差异[J]. 农药学学报, 2000, 2(4):44-48.
[31] 成燕清, 王培, 唐涛, 等. 小菜蛾对氯虫苯甲酰胺抗药性与田间防治效果的相关性研究[J]. 应用昆虫学报, 2016, 53(2):320-324.
[32] 沐卫东, 尹艳球, 黄春芬, 等. 通海小菜蛾田间种群发生及其对9种杀虫剂的抗药性[J]. 中国植保导刊, 2014, 34(9):13-16, 52.
[33] 梁延坡, 谢圣华, 符尚娇, 等. 海南地区小菜蛾对氯虫苯甲酰胺和乙基多杀菌素的抗药性变化趋势[J]. 应用昆虫学报, 2016, 53(2):273-278.
[34] Zhou L J, Huang J G, Xu H H. Monitoring resistance of field populations of diamondback moth Plutell axylostella L. (Lepidoptera:Yponomeutidae) to five insecticides in south China:a ten-year case study[J]. Crop Protection, 2011, 30(3):272-278.
[35] 李振宇, 陈焕瑜, 包华理, 等. 小菜蛾区域性抗药性治理技术研究——公益性行业(农业)科研专项"十字花科小菜蛾综合防控技术研究与示范推广"研究进展[J]. 应用昆虫学报, 2016, 53(2):247-255.
[36] 尹艳琼, 李向永, 赵雪晴, 等. 云南不同菜区小菜蛾对三种生物农药的抗药性及其变化趋势[J]. 应用昆虫学报, 2016, 53(2):285-291.
[37] Guo Z J, Kang S, Chen D F, et al. The MAPK signaling pathway alters expression of midgut receptor genes and confers resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback moth[J]. PLoS Genetics, 2015, 11(4):e1005124.
[38] 吴刚, 尤民生, 赵士熙. 小菜蛾抗性稳定性及抗性治理对策研究[J]. 农药学学报, 2001, 3(1):83-86.