Effect of Temperature on Trypsin Activity and Morphology of Digestive Organs of Arma chinensis (Hemiptera: Pentatomidae)

doi:10.16409/j.cnki.2095-039x.2021.05.007

Abstract

Abstract: Proteases play important roles in food digestion and growth and development in predatory insects. The physiological mechanism of digestion and the optimal period for field release in different seasons were investigated through measuring and comparing trypsin enzyme activities of adults of Arma chinensis at five temperatures 15℃, 20℃, 26℃, 30℃, and 35℃. A double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to determine the activities of trypsin in the current study. Trypsin activity concentrations in salivary glands and midgut of A. chinensis were calculated according to standard curve formula. Meanwhile, morphology and structure of oral organs, salivary glands and digestive tract of A. chinensis were preliminarily observed. The results showed that the trypsin activity in A. chinensis varied between temperatures, and the enzyme activity in the salivary glands was much higher than that in the midgut. At 26℃, the enzyme activities reached the highest in both the salivary glands and the midgut, which were 1413.9 IU/L and 1029.2 IU/L, respectively, and the difference between them reached the most. The proboscis of A. chinensis consists of four joints. The salivary glands consist of a pair of main salivary glands and a pair of accessory salivary glands.

Key words: Arma chinensis, temperature, trypsin activity, salivary glands, mouthparts

CLC Number:

S476.2

ZHU Yanjuan, YIN Yanfang, WANG Zhen, CHEN Hongyin, LIU Chenxi. Effect of Temperature on Trypsin Activity and Morphology of Digestive Organs of Arma chinensis (Hemiptera: Pentatomidae)[J]. Chinese Journal of Biological Control, 2022, 38(3): 767-773.

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