Resistance Development of Four Bt Insecticidal Crystal Proteins against Btk-resistant Strain of Plutella xylostella in the Laboratory

doi:10.16409/j.cnki.2095-039x.2021.05.019

Abstract

Abstract: The Bacillus thuringiensis (Bt)-resistant strains of Plutella xylostella (L.) were selected using a wettable powder formulation of Bt var. kurstaki (Btk) for 80 and 100 generations in the laboratory, respectively. The toxicities of four Bt-insecticidal crystal proteins (Cry1Ab, Cry1Ac, Cry1Ah, and Cry1Ca) against Btk-resistant and -susceptible strains of P. xylostella were determined using a standard leaf-dipping method. For the F₈₀ generation of Btk-resistant strain, Cry1Ca had higher toxicity with a LC₅₀value about 12.1 mg/Lthan the other three Cry proteins, followed by Cry1Ac with a LC₅₀value about 47.7 mg/L. For the F₁₀₀ generation of Btk-resistant strain, Cry1Ca still showed higher toxicity with a LC₅₀ value about 21.4 mg/Lthan the other three Cry proteins, followed by Cry1Ab with a LC₅₀ value about 72.2 mg/L. The Btk-resistant strains of P. xylostella showed quick development of resistance to Cry1Ac, with a resistance ratio (RR) increased from 67.3-fold to 106.8-fold when compared with the susceptible laboratory strain. The resistance to Cry1Ab was relatively low, with a RR value from 60.0-fold to 66.1-fold, and the resistance to Cry1Ca and Cry1Ah was even lower, with RR values of 3.4—6.0-fold and 1.6—2.5-fold, respectively. In conclusion, these results suggest that, under the selection pressure of Btk with a dominant gene of Cry1Ac, P. xylostella strains show significant differences in the development of resistance to four insecticidal crystal proteins, and that there is a potential risk of cross-resistance between Cry1Ac and Cry1Ab.

Key words: Plutella xylostella, Bacillus thuringiensis, insecticidal crystal protein, bioassay, resistance

CLC Number:

S476.1

FU Wei, TANG Tao, WANG Pei, WANG Yong, YIN Li, MA Mingyong, ZHANG Zhengbing. Resistance Development of Four Bt Insecticidal Crystal Proteins against Btk-resistant Strain of Plutella xylostella in the Laboratory[J]. Chinese Journal of Biological Control, 2022, 38(2): 328-332.

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