Xylo-oligosaccharides Elicits Disease Resistance in Arabidopsis

doi:10.16409/j.cnki.2095-039x.2021.01.030

Abstract

Abstract: Plant-origined xylo-oligosaccharides (XOS) have many excellent physiological and biological properties in animals. However, whether XOS acts as an oligosaccharins to induce plant defense remains unclear. In this study, the induced disease resistance to different pathogens upon XOS with different concentrations was investigated. Here, we found that XOS can induce plant resistance against hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000, biotrophic pathogen Tobacco mosaic virus and necrotrophic pathogen Sclerotinia sclerotiorum in a dose-dependent manner. The induced resistance effect to Pst DC3000 and TMV increased as XOS concentration increased to 100 μg/mL. While XOS concentration increased to 50 μg/mL, the induced resistance effect to S. sclerotiorum was no longer rising. The results indicated that another new biotic sugar, XOS, can broadly elicit disease resistance in Arabidopsis, which provide a basis for elucidating the signal transduction of disease resistance induced by XOS and designing efficient strategies for plant disease control.

Key words: xylo-oligosaccharides, oligosaccharins, disease resistance, pathogen

CLC Number:

S476

MIU Jinlu, ZHANG Huajian. Xylo-oligosaccharides Elicits Disease Resistance in Arabidopsis[J]. Chinese Journal of Biological Control, 2021, 37(1): 150-155.

References

[1] Zhang W, Zhao F, Jiang L, et al. Different pathogen defense strategies in Arabidopsis:more than pathogen recognition[J]. Cells, 2018, 7(12):252.
[2] Rocafort M, Fudal I, Mesarich C H. Apoplastic effector proteins of plant-associated fungi and oomycetes[J]. Current Opinion in Plant Biology, 2020, 56:9-19.
[3] Fonseca J P, Mysore K S. Genes involved in nonhost disease resistance as a key to engineer durable resistance in crops[J]. Plant Science, 2019, 279:108-116.
[4] Ingle R A, Carstens M, Denby K J. PAMP recognition and the plant-pathogen arms race[J]. Bioessays, 2006, 28(9):880-889.
[5] Garcia-Brugger A, Lamotte O, Vandelle E, et al. Early signaling events induced by elicitors of plant defenses[J]. Molecular Plant-Microbe Interactions, 2006, 19(7):711-724.
[6] Darvill A G, Albersheim P, McNeil M, et al. Structure and function of plant cell wall polysaccharides[J]. Journal of Cell Science, 1985, 2(S):203-217.
[7] Gravino M, Savatin D V, Macone A, et al. Ethylene production in Botrytis cinerea- and oligogalacturonide-induced immunity requires calcium-dependent protein kinases[J]. Plant Journal, 2015, 84(6):1073-1086.
[8] Brutus A, Sicilia F, Macone A, et al. A domain swap approach reveals a role of the plant wall-associated kinase 1(WAK1) as a receptor of oligogalacturonides[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(20):9452-9457.
[9] Sharp J K, Valent B, Albersheim P. Purification and partial characterization of a beta-glucan fragment that elicits phytoalexin accumulation in soybean[J]. Journal of Biological Chemistry, 1984, 259(18):11312-11320.
[10] Sharp J K, McNeil M, Albersheim P. The primary structures of one elicitor-active and seven elicitor-inactive hexa(beta-D-glucopyranosyl)-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f. sp. glycinea[J]. Journal of Biological Chemistry, 1984, 259(18):11321-11336.
[11] Yamaguchi T, Yamada A, Hong N, et al. Differences in the recognition of glucan elicitor signals between rice and soybean:beta-glucan fragments from the rice blast disease fungus Pyricularia oryzae that elicit phytoalexin biosynthesis in suspension-cultured rice cells[J]. Plant Cell, 2000, 12(5):817-826.
[12] Brule D, Villano C, Davies L J, et al. The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide- triggered immunity[J]. Plant Biotechnology Journal, 2018, 17(4):812-825.
[13] Azevedo Carvalho A F, de Oliva Neto P, Da Silva D F, et al. Xylo-oligosaccharides from lignocellulosic materials:Chemical structure, health benefits and production by chemical and enzymatic hydrolysis[J]. Food Research International, 2013, 51:75-85.
[14] Aachary A A, Prapulla S G. Xylooligosaccharides (XOS) as an emerging prebiotic:microbial synthesis, utilization, structural characterization, bioactive properties, and applications[J]. Comprehensive Reviews in Food Science and Food Safety, 2011, 10:2-16.
[15] Broekaert W F, Courtin C M, Verbeke K, et al. Prebiotic and other health-related effects of cereal-derived arabinoxylans, arabinoxylan-oligosaccharides, and xylooligosaccharides[J]. Critical Reviews in Food Science and Nutrition, 2011, 51(2):178-194.
[16] Yuan L, Li W, Huo Q, et al. Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens[J]. Peerj, 2018, 6:e4435.
[17] Gibson G R. From probiotics to prebiotics and a healthy digestive system[J]. Journal of Food Science, 2004, 69(5):141-143.
[18] Nabarlatz D, Montane D, Kardosova A, et al. Almond shell xylo-oligosaccharides exhibiting immunostimulatory activity[J]. Carbohydrate Research, 2007, 342(8):1122-1128.
[19] Chen W, Guo C, Hussain S, et al. Role of xylo-oligosaccharides in protection against salinity-induced adversities in Chinese cabbage[J]. Environmental Science and Pollution Research, 2016, 23(2):1254-1264.
[20] Steffen J G, Kang I H, Portereiko M F, et al. AGL61 interacts with AGL80 and is required for central cell development in Arabidopsis[J]. Plant Physiology, 2008, 148(1):259-268.
[21] Htet A N, Noguchi M, Ninomiya K, et al. Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid[J]. Journal of Bioscience and Bioengineering, 2018, 125(6):717-722.
[22] Chen L, Zhang S J, Zhang S S, et al. A fragment of the Xanthomonas oryzae pv. oryzicola harpin HpaG (Xooc) reduces disease and increases yield of rice in extensive grower plantings[J]. Phytopathology, 2008, 98(7):792-802.
[23] Le M H, Cao Y, Zhang X C, et al. LIK1, a cerk1-interacting kinase, regulates plant immune responses in Arabidopsis[J]. Plos One, 2014, 9(7):e102245.
[24] Jia X, Meng Q, Zeng H, et al. Chitosan oligosaccharide induces resistance to tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway[J]. Scientific Reports, 2016, 6:26144.
[25] Jia X, Zeng H, Wang W, et al. Chitosan oligosaccharide induces resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana by activating both salicylic acid- and jasmonic acid-mediated pathways[J]. Molecular Plant-Microbe Interactions, 2018, 31(12):1271-1279.
[26] Liu J, Zhang X, Kennedy J F, et al. Chitosan induces resistance to tuber rot in stored potato caused by Alternaria tenuissima[J]. International Journal of Biological Macromolecules, 2019, 140:851-857.