Isolation and Identification of Oat Endophytic Bacteria YN-J3 and Study on Its Growth Promotion and Disease Control Effects

doi:10.16409/j.cnki.2095-039x.2022.02.015

Abstract

Abstract: Plant endophytes are important biocontrol resources. In this study, the endophytic bacteria of oat were isolated by surface sterilization and gradient dilution coating method, and the bioassay of endophytic bacteria was also conducted. A total of 512 endophytic bacteria strains were isolated from 52 oat samples in different sites, among which 9 bacteria strains could significantly inhibited mycelium growth of Colletotrichum graminicola and Drechslera avenae causing oat leaf spot. Moreover, strain YN-J3 had the best antifungal effect to both pathogens. The control efficacies of strain YN-J3 were 75.27%, 74.53% and 63.57% in vitro and field experiments, respectively. The green house experiment showed that YN-J3 significantly promoted oat growth. The biochemical test showed that YN-J3 had the functions of dissolving phosphorus, dissolving potassium, and producing IAA. YN-J3 was identified as Bacillus amyloliquefaciens. These results indicated that B. amyloliquefaciens YN-J3 was important biological resource of oat in controlling leaf spot and promoting growth.

Key words: endophytic bacteria, growth-promoting, antagonistic, bioassay

CLC Number:

S476

WANG Haixia, ZHENG Chengzhong, DONG Baozhu, MENG Huanwen, ZHOU Hongyou, SUN Ruifeng, ZHENG Hongli. Isolation and Identification of Oat Endophytic Bacteria YN-J3 and Study on Its Growth Promotion and Disease Control Effects[J]. Chinese Journal of Biological Control, 2022, 38(2): 447-457.

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