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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (2): 349-359.DOI: 10.16409/j.cnki.2095-039x.2022.03.002

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Compatibility of Metiarium flavoride with Trans-Anethole and Their Cotoxicity to Pieris rapae

LI Lei, ZHANG Lei, ZHAO Zongxiang, WANG Mengru, LI Shiguang   

  1. College of Plant Protection, Anhui Agricultural University/Key Laboratory of Plant Diseases and Insect Pest Biology and Green Prevention and Control in Universities of Anhui Province, Hefei 230036, China
  • Received:2021-01-20 Online:2022-04-08 Published:2022-04-20

Abstract: The compatibility of plant secondary metabolite trans-anethole with a highly virulent entomopathogenic fungi Metarhizium flavoride Mf82 were evaluated using mixtures of LC50 of trans-anethole and different concentrations of conidial suspensions of M. flavoride Mf82 at different ratios in terms of the colony growth of M. flavoride and their co-toxicity against the 2nd instar larvae of Pieris rapae in the laboratory. Results indicated that each of the two showed good insecticidal activities against the 2nd instar larvae of P. rapae. The mixture of the two promoted the mycelia growth of M. flavoride to some extent, the effect was significant when the mixture ratios were at 5:5, 4:6 and 3:7 and was even significant as the proportion of trans-anethole in the mixture increased. The conidia germination rate was also higher than in the mixtures mixed at 2:8 and 1:9. The mortalities of the 2nd instar larvae of P. rapae treated with the mixtures varied with the mixing ratios, the mixtures of the two at mixing ratios of 5:5, 4:6 and 3:7 resulted in higher mortalities than that obtained with the mixtures mixed at 2:8 and 1:9, with co-toxicity coefficients of 287, 108 and 83, respectively, indicating synergistic and additive effects. The LT50values of the mixtures mixed at 5:5, 4:6 and 3:7 were smaller than that of M. flavoride alone. In the control practice of P. rapae, it is recommended to use the mixing ratios with good compatibility and high co-toxicity coefficient of the two agents, that is, trans-anethole (LC50) and M. flavoride Mf82 mixed at 5:5, 4:6 and 3:7. The high cotoxicity of the combination of M. flavoride with trans-anethole indicates that the mixture can be a potential alternative to the chemicals as an effective component of IPM program against P. rapae.

Key words: Metarhizium flavoride, trans-anethole, Pieris rapae, biological control, cotoxicity

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