Optimization of Fermentation Medium for the Production of Surfactin by Engineered Strain B9BD of Bacillus subtilis

doi:10.16409/j.cnki.2095-039x.2022.02.006

Abstract

Abstract: Bacillus subtilis Bs916 is the main component of the biopesticide “Wenquning”, which can produce a variety of lipopeptide antibiotics, such as Surfactin, Fengycin and Iturin. In a previous study, an engineering strain B9∆degU∆bmyA (B9BD) was found to be highly efficient in the synthesis of Surfactin. In this study, Plackett-Burman experiment, central xomposite design and response surface methodology were used to optimize the fermentation medium components of strain B9BD for Surfactin production. The optimized fermentation medium for high-yield Surfactin of strain B9BD was: glucose 24.27 g/L, soybean meal 29.05 g/L, beef extract 3 g/L, NH₄NO₃ 4 g/L, L-Leucine 2.36 g/L, KH₂PO₄ 3 g/L, NaCl 3 g/L and FeSO₄ 0.02 g/L. After optimization, the highest production of Surfactin reached (934.25±32.67) mg/L, which was 5.6 times that of the LB medium. The above research results will provide the theoretical basis and technical support for the efficient fermentation and application of Surfactin.

Key words: Bacillus subtilis, Surfactin, Plackett-Burman experiment, central composite design, response surface methodology

CLC Number:

S476

QIAO Junqing, SUN Kai, LIU Yongfeng, CHEN Xijun, LIU Youzhou. Optimization of Fermentation Medium for the Production of Surfactin by Engineered Strain B9BD of Bacillus subtilis[J]. Chinese Journal of Biological Control, 2022, 38(2): 383-392.

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