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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (5): 1083-1093.DOI: 10.16409/j.cnki.2095-039x.2022.03.030

• RESEARCH REPORTS • Previous Articles     Next Articles

Effect of the Global Regulator MtrAsbh on the Biosynthesis of Milbemycins

ZHU Yajie1, WANG Jiabin1,2, LI Shanshan1, XIANG Wensheng1,2, ZHANG Yanyan1   

  1. 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. School of Life Science, Northeast Agricultural University, Harbin 150030, China
  • Received:2022-05-23 Online:2023-10-08 Published:2023-10-25

Abstract: MtrA is a global response regulatory protein that is highly conserved in Streptomyces. The function of MtrA from Streptomyces bingchenggensis (MtrAsbh) in the biosynthesis of biopesticide milbemycins was studied by by gene deletion, complementation, overexpression, transcriptional analysis and gel retardation assay. Results showed that the mtrAsbh deletion mutant displayed a complete loss of milbemycin production, while overexpression of mtrAsbh significantly promoted the production of milbemycin, indicating that MtrAsbh is a key activator of milbemycin biosynthesis. Our results further show that MtrAsbh regulates milbemycin production by affecting the expression of milbemycin biosynthetic gene clusters and genes related to precursor supply. In addition, MtrAsbhcan act as an activator or a repressor to differentially control the expression of many other natural product biosynthesis genes presented in the genome of Streptomyces bingchenggensis.

Key words: global regulator, MtrAsbh, Streptomyces bingchenggensis, milbemycin

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