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Chinese Journal of Biological Control ›› 2024, Vol. 40 ›› Issue (2): 484-490.DOI: 10.16409/j.cnki.2095-039x.2023.01.025

• SCIENTIFIC NOTES • Previous Articles    

Identification and Field Control Efficiency of High Virulence Bt Strain against Phyllotreta striolata

BIAN Qiang1, YU Shujing1, SUN Xiaodong2   

  1. 1. College of Chemistry, National Pesticide Engineering Research Center(Tianjin), Nankai University, Tianjin 300071, China;
    2. The Institute of Vegetables, Hainan Academy of Agricultural Sciences/Hainan Key Laboratory of Vegetable Biology, Haikou 571100, China
  • Received:2022-12-27 Published:2024-04-28

Abstract: Biological control of Phyllotreta striolata was explored by isolating and screening highly virulent Bt strains.A strain was isolated from the soil of Hainan Province,which was identified using morphological and molecular methods as a Bacillus thuringiensis strain and named as BS128,and the strain contained a novel insecticidal coding gene Cry8Ga.Laboratory assay of the strain showed a 3-d LC50 of 60.0093 μg/mL against P.striolata.Field tests showed a control efficiency of 67.66% against P.striolata at 15 d post-treatment using 250 μg/mL BS128 suspension 9000 mL/hm2 as soil treatment and control efficacies of 61.86% and 62.16% against P.striolata at 7 d and 10 d post-treatment using 250 μg/mL BS128 suspension 9000 mL/hm2 as foliar spray,respectively.The results indicate that soil treatment using the strain achieved equal control of P.striolata to that of foliar spray.We believe that soil treatment using the strain is more suitable for control of P.striolata,because the soil treatment using the strain against P.striolata larvae in the soil can lessen the damage of adult beetles in the seedling stage and,Bt is more effective in the soil and has a longer persistence,which avoids the inactivation of foliar sprayed Bt by high temperature or strong UV radiation.

Key words: Phyllotreta striolata, Bacillus thuringiensis, bioassay, field control efficiency

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